Characterisation of rhizobia from African acacias and other tropical woody legumes using Biolog™ and partial 16S rRNA sequencing

被引：28

作者：

McInroy, SG ^{[1
]}

Campbell, CD

Haukka, KE

Odee, DW

Sprent, JI

Wang, WJ

Young, JPW

Sutherland, JM

机构：

[1] Univ Dundee, Dept Biol Sci, Dundee DD1 4HN, Scotland

[2] Macaulay Land Use Res Inst, Soil Sci Grp, Aberdeen AB15 8QH, Scotland

[3] Univ York, Dept Biol, York YO1 5YW, N Yorkshire, England

[4] Kenya Forestry Res Inst, Div Biotechnol, Nairobi, Kenya

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 170卷 / 01期

关键词：

tropical Rhizobium; Biolog; African acacia; characterisation; diversity; legume;

D O I：

10.1111/j.1574-6968.1999.tb13362.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Biolog(TM) (sole carbon source utilisation) user database of tropical and temperature rhizobial strains was created and used in conjunction with the partial 16S rRNA sequencing method to characterise 12 rhizobial isolates from African acacias and other tropical woody legumes. There was close agreement between the two methods but also some significant discrepancies. A high degree of diversity was shown in the relatively small sample of isolates, with 4 out of 5 of the currently proposed rhizobial genera represented. This is the first time Biolog has shown congruence with genotypic fingerprinting: using a wide selection of rhizobial reference and test strains. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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收藏

页码：111 / 117

页数：7

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