Modulation of secretory granule-targeting efficiency by cis and trans compounding of sorting signals

被引:15
作者
Lacombe, MJ [1 ]
Mercure, C [1 ]
Dikeakos, JD [1 ]
Reudelhuber, TL [1 ]
机构
[1] Clin Res Inst Montreal, Lab Mol Biochem Hypertens, Montreal, PQ H2W 1R7, Canada
关键词
D O I
10.1074/jbc.M408658200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several protein domains acting through seemingly different mechanisms have been reported to have the capacity to target proteins to dense core secretory granules. Because proteins enter secretory granules with different efficiencies and because some of these proteins contain more than one granule-targeting motif, we have investigated whether compounding sorting signals could alter the efficiency of protein entry into secretory granules. In the current study we demonstrate that a paired basic cleavage site from human prorenin and an a-helix-containing secretory granule-sorting signal from the prohormone convertase PC1/3 can synergize to increase granule-sorting efficiency not only when located on the same protein, but also when located on distinct proteins that associate in the secretory pathway.
引用
收藏
页码:4803 / 4807
页数:5
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