Dumbbell-shaped nanocircular RNAs for RNA interference

被引：65

作者：

Abe, Naoko ^{[1
]}

Abe, Hiroshi ^{[1
]}

Ito, Yoshihiro ^{[1
]}

机构：

[1] RIKEN, Discovery Res Inst, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 49期

关键词：

D O I：

10.1021/ja0754453

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops. RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.

引用

页码：15108 / +

页数：3

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