Cloned and expressed rat Ca2+-sensing receptor - Differential cooperative responses to calcium and magnesium

被引：115

作者：

Ruat, M

Snowman, AM

Hester, LD

Snyder, SH

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC SCI,BALTIMORE,MD 21205

[4] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21205

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 11期

关键词：

D O I：

10.1074/jbc.271.11.5972

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have stably expressed cDNA for the rat brain Ca2+-sensing receptor in Chinese hamster ovary cells. Stimulation of phosphatidylinositol hydrolysis and arachidonic acid (AA) release displayed markedly cooperative responses to Ca2+ with Hill coefficients of 4-5. Both phosphatidylinositol and AA responses were not detected below a threshold of 1.5 mM Ca2+. Mg2+ behaved as a partial agonist with only half the maximal inositol phosphate and AA responses displayed by Ca2+ and with a more shallow concentration-response slope. The potency of Mg2+ in augmenting inositol phosphate and AA responses, in the presence of 1.5 mM Ca2+, implies that serum Mg2+ concentrations attained in clinical conditions will influence the Ca2+-sensing receptor.

引用

页码：5972 / 5975

页数：4

共 18 条

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