Determination of a new antimalarial drug, benflumetol, in blood plasma by high-performance liquid chromatography

被引：39

作者：

Mansor, SM

Navaratnam, V

Yahaya, N

Nair, NK

Wernsdorfer, WH

Degen, PH

机构：

[1] UNIV SAINS MALAYSIA,CTR DRUG RES,MINDEN 11800,PENANG,MALAYSIA

[2] UNIV VIENNA,INST SPECIF PROPHYLAXIS & TROP MED,A-1095 VIENNA,AUSTRIA

[3] CIBA GEIGY LTD,BIOANALYT & PHARMACOKINET,CH-4002 BASEL,SWITZERLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 682卷 / 02期

关键词：

benflumetol;

D O I：

10.1016/0378-4347(96)00086-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and selective high-performance liquid chromatographic assay for determination of a new antimalarial drug (benflumetol, BFL) is described. After extraction with hexane-diethyl ether (70:30, v/v) from plasma, BFL was analysed using a C-18 Partisil 10 ODS-3 reversed-phase stainless steel column and a mobile phase of acetonitrile-0.1 M ammonium acetate (90:10, v/v) adjusted to pH 4.9 with ultraviolet detection at 335 nm. The mean recovery of BFL over a concentration range of 50-400 ng/ml was 96.8+/-5.2%. The within-day and day-to-day coefficients of variation were 1.8-4.0 and 1.8-4.2%, respectively. The minimum detectable concentration in plasma for BFL was 5 ng/ml with a C.V. of less than 10%. This method was found to be suitable for clinical pharmacokinetic studies.

引用

页码：321 / 325

页数：5