Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci

被引:37
作者
Paule, SM
Trick, WE
Tenover, FC
Lankford, M
Cunningham, S
Stosor, V
Cordell, RL
Peterson, LR
机构
[1] Northwestern Univ, NW Mem Hosp, Evanston, IL 60201 USA
[2] Northwestern Univ, Feinberg Sch Med, Evanston, IL 60201 USA
[3] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Natl Ctr Infect Dis, Atlanta, GA USA
关键词
D O I
10.1128/JCM.41.10.4805-4807.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46; P < 0.001) or perianal swab (17 of 58 versus 12 of 58; P = 0.059) for the detection of gastrointestinal colonization by vancomycin-resistant enterococci.
引用
收藏
页码:4805 / 4807
页数:3
相关论文
共 15 条
[1]   A hospital epidemic of vancomycin-resistant Enterococcus:: Risk factors and control [J].
Byers, KE ;
Anglim, AM ;
Anneski, CJ ;
Germanson, TP ;
Gold, HS ;
Durbin, LJ ;
Simonton, BM ;
Farr, BM .
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2001, 22 (03) :140-147
[2]   High rate of false-negative results of the rectal swab culture method in detection of gastrointestinal colonization with vancomycin-resistant enterococci [J].
D'Agata, EMC ;
Gautam, S ;
Green, WK ;
Tang, YW .
CLINICAL INFECTIOUS DISEASES, 2002, 34 (02) :167-172
[3]   Effect of antibiotic therapy on the density of vancomycin-resistant enterococci in the stool of colonized patients. [J].
Donskey, CJ ;
Chowdhry, TK ;
Hecker, MT ;
Hoyen, CK ;
Hanrahan, JA ;
Hujer, AM ;
Hutton-Thomas, RA ;
Whalen, CC ;
Bonomo, RA ;
Rice, LB .
NEW ENGLAND JOURNAL OF MEDICINE, 2000, 343 (26) :1925-1932
[4]   DETECTION OF GLYCOPEPTIDE RESISTANCE GENOTYPES AND IDENTIFICATION TO THE SPECIES LEVEL OF CLINICALLY RELEVANT ENTEROCOCCI BY PCR [J].
DUTKAMALEN, S ;
EVERS, S ;
COURVALIN, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :24-27
[5]  
Facklam R.R.S., 1999, Manual of Clinical Microbiology, P297
[6]   Insights into the epidemiology and control of infection with vancomycin-resistant enterococci [J].
Hayden, MK .
CLINICAL INFECTIOUS DISEASES, 2000, 31 (04) :1058-1065
[7]   Control of vancomycin-resistant enterococci at a community hospital: Efficacy of patient and staff cohorting [J].
Jochimsen, EM ;
Fish, L ;
Manning, K ;
Young, S ;
Singer, DA ;
Baker, R ;
Jarvis, WR .
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 1999, 20 (02) :106-109
[8]   Comparison of five selective media for identifying fecal carriage of vancomycin-resistant enterococci [J].
Landman, D ;
Quale, JM ;
Oydna, E ;
Willey, B ;
Ditore, V ;
Zaman, M ;
Patel, K ;
Saurina, G ;
Huang, W .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (03) :751-752
[9]  
National Committee for Clinical Laboratory Standards, 2000, METH DIL ANT SUSC TE
[10]   Control of vancomycin-resistant enterococcus in health care facilities in a region. [J].
Ostrowsky, BE ;
Trick, WE ;
Sohn, AH ;
Quirk, SB ;
Holt, S ;
Carson, LA ;
Hill, BC ;
Arduino, MJ ;
Kuehnert, MJ ;
Jarvis, WR .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 344 (19) :1427-1433