Vibrational imaging with high sensitivity via epidetected coherent anti-Stokes Raman scattering microscopy

被引：298

作者：

Volkmer, A ^{[1
]}

Cheng, JX ^{[1
]}

Xie, XS ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

PHYSICAL REVIEW LETTERS | 2001年 / 87卷 / 02期

关键词：

D O I：

10.1103/PhysRevLett.87.023901

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We demonstrate theoretically and experimentally a novel epidetection scheme for coherent anti-Stokes Raman scattering (CARS) microscopy that significantly improves the detection sensitivity. Calculations show that epidetected CARS (E-CARS) signals are present for scatterers smaller than the wavelength of light, whereas the large background signals from the:surrounding bulk solvent are suppressed by destructive interference. E-CARS microscopy is capable of revealing small intracellular features that are otherwise buried by the strong water CARS signal.

引用

页数：4

共 18 条

[1] Nonlinear scanning laser microscopy by third harmonic generation [J].