Retroviral display and high throughput screening

被引：14

作者：

Buchholz, Christian J. ^{[1
]}

Duerner, Lydia J. ^{[1
]}

Funke, Sabrina ^{[1
]}

Schneider, Irene C. ^{[1
]}

机构：

[1] Paul Ehrlich Inst, Div Med Biotechnol, D-63225 Langen, Germany

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2008年 / 11卷 / 02期

关键词：

directed evolution; antibody; antigen; protease substrate; retroviral vector; protein engineering;

D O I：

10.2174/138620708783744543

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Retroviruses distinguish themselves from all other mammalian viruses by their abilities to infect and propagate in mammalian cells without causing a cytopathic effect and to stably integrate their genetic information into the genome of the host cell. These unique properties make them an ideal platform for the display and directed evolution of proteins in a mammalian cell environment. This review will describe the essentials about retrovirus biology and then discuss in detail display and screening strategies that have been developed during the past 15 years of retroviral display technology.

引用

页码：99 / 110

页数：12

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