Comparison of intensity distributions in tomograms from BF TEM, ADF STEM, HAADF STEM, and calculated tilt series

被引:55
作者
Friedrich, H [1 ]
McCartney, MR
Buseck, PR
机构
[1] Arizona State Univ, Dept Geol Sci, Tempe, AZ 85287 USA
[2] Arizona State Univ, Dept Chem Biochem, Tempe, AZ 85287 USA
[3] Arizona State Univ, Dept Phys & Astron, Tempe, AZ 85287 USA
基金
美国国家科学基金会; 美国国家航空航天局;
关键词
electron tomography; bright-field TEM imaging; HAADF STEM imaging; histogram analysis; magnetite crystals;
D O I
10.1016/j.ultramic.2005.06.005
中图分类号
TH742 [显微镜];
学科分类号
摘要
The three-dimensional (3D) morphology of a nanometer-sized object can be obtained using electron tomography. Variations in composition or density of the object cause variations in the reconstructed intensity. When imaging homogeneous objects, variations in reconstructed intensity are caused by the imaging technique, imaging conditions, and reconstruction. In this paper, we describe data acquisition, image processing, and 3D reconstruction to obtain and compare tomograms of magnetite crystals from bright field (BF) transmission electron microscopy (TEM), annular dark-field (ADF) scanning transmission electron microscopy (STEM), and high-angle annular dark field (HAADF) STEM tilt series. We use histograms, which plot the number of volume elements (voxels) at a given intensity vs. the intensity, to measure and quantitatively compare intensity distributions among different tomograms. In combination with numerical simulations, we determine the influence of maximum tilt angle, tilt increment, contrast changes with tilt (diffraction contrast), and the signal-to-noise ratio (SNR) as well as the choice of the reconstruction approach (weighted backprojection (WB) and sequential iterative reconstruction technique (SIRT)) on the histogram. We conclude that because ADF and HAADF STEM techniques are less affected by diffraction, and because they have a higher SNR than BF TEM, they are better suited for tomography of nanometer-sized crystals. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:18 / 27
页数:10
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