Fluorescence-lifetime technologies for high-throughput screening

被引：9

作者：

French, T ^{[1
]}

Owicki, JC ^{[1
]}

Modlin, DN ^{[1
]}

Deshpande, SS ^{[1
]}

Mineyev, I ^{[1
]}

Crawford, K ^{[1
]}

Burton, W ^{[1
]}

机构：

[1] LJL BioSyst Inc, Sunnyvale, CA 94089 USA

来源：

SYSTEMS AND TECHNOLOGIES FOR CLINICAL DIAGNOSTICS AND DRUG DISCOVERY, PROCEEDINGS OF | 1998年 / 3259卷

关键词：

drug discovery; background rejection; ruthenium; phase-sensitive; phase-resolved; fluorescence spectroscopy;

D O I：

10.1117/12.307330

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Fluorescence lifetime, the mean interval between absorption and emission, is as fundamental a characteristic of fluorescence as excitation and emission wavelengths and quantum yield. Yet, with the exception of time-resolved fluorescence assays utilizing lanthanide chelates, the analytical possibilities of methods based on fluorescence lifetime are virtually unexploited outside the academic research laboratory. We discuss the potential use of fluorescence-lifetime technologies in high-throughput screening from the standpoint of assay reagents and instrumentation. Among these applications are fluorescence-polarization assays based on long-lifetime probes and fluorescence-intensity assays using lifetime-resolved detection to reject background. We find that fluorescence-lifetime technologies offer significant practical advantages over existing methods.

引用

页码：209 / 218

页数：10