Stage-dependent production and release of histidine-rich protein 2 by Plasmodium falciparum

被引:83
作者
Desakorn, V
Dondorp, AM
Silamut, K
Pongtavornpinyo, W
Sahassananda, D
Chotivanich, K
Pitisuttithum, P
Smithyman, AM
Day, NPJ
White, NJ
机构
[1] Mahidol Univ, Fac Trop Med, Bangkok 10400, Thailand
[2] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Ctr Trop Med, Oxford OX1 2JD, England
[3] Cellabs Pty Ltd, Brookvale, NSW 2100, Australia
基金
英国惠康基金;
关键词
Plasmodium falciparum; histidine-rich protein 2; staging; stage-dependent production; parasite biomass;
D O I
10.1016/j.trstmh.2004.11.014
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Because of their sequestration in the microcirculation, the pathogenic late stages of Plasmodium falciparum are under-represented in peripheral blood samples from patients with falciparum malaria. Excreted products of the parasite might help to estimate this sequestered biomass. We quantified the stage-dependent production and release per parasite of P falciparum histidine-rich protein 2 (PfHRP2) with the objective of measuring the sequestered biomass. A simple method to relate parasite stage to parasite age was developed to facilitate this. In four isolates of P falciparum, the median (range) PfHRP2 content was 2.0 fg (0.5-4.3fg) for a young ring stage infected erythrocyte, and 5.4 fg (2.1-10.2 fg) for the schizont stage. The amount of PfHRP2 in the parasitized erythrocyte increased most during development to the mature trophozoite stage. The median (range) amount of PfHRP2 secreted per parasite per entire erythrocytic cycle was 5.2 fg (1.1-13.0 fg). A median of 89% of the total PfHRP2 was excreted at the moment of schizont rupture. This assessment of the stage-dependent release of PfHRP2 is an essential prerequisite for future studies aimed at estimating the total patient parasite mass from the peripheral blood PfHRP2 concentration. (c) 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:517 / 524
页数:8
相关论文
共 17 条
[1]   Spectroscopic characterization of the heme-binding sites in Plasmodium falciparum histidine-rich protein 2 [J].
Choi, CYH ;
Cerda, JF ;
Chu, HA ;
Babcock, GT ;
Marletta, MA .
BIOCHEMISTRY, 1999, 38 (51) :16916-16924
[2]   Semi-quantitative measurement of Plasmodium falciparum antigen PfHRP2 in blood and plasma [J].
Desakorn, V ;
Silamut, K ;
Angus, B ;
Sahassananda, D ;
Chotivanich, K ;
Suntharasamai, P ;
Simpson, J ;
White, NJ .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1997, 91 (04) :479-483
[3]  
Genton Blaise, 1998, Southeast Asian Journal of Tropical Medicine and Public Health, V29, P35
[4]   SECRETION OF A MALARIAL HISTIDINE RICH PROTEIN (PFHRP-II) FROM PLASMODIUM-FALCIPARUM INFECTED ERYTHROCYTES [J].
HOWARD, RJ ;
UNI, S ;
AIKAWA, M ;
ALEY, SB ;
LEECH, JH ;
LEW, AM ;
WELLEMS, TE ;
RENER, J ;
TAYLOR, DW .
JOURNAL OF CELL BIOLOGY, 1986, 103 (04) :1269-1277
[5]   SYNCHRONIZATION OF PLASMODIUM-FALCIPARUM ERYTHROCYTIC STAGES IN CULTURE [J].
LAMBROS, C ;
VANDERBERG, JP .
JOURNAL OF PARASITOLOGY, 1979, 65 (03) :418-420
[6]   INDICATORS OF LIFE-THREATENING MALARIA IN AFRICAN CHILDREN [J].
MARSH, K ;
FORSTER, D ;
WARUIRU, C ;
MWANGI, I ;
WINSTANLEY, M ;
MARSH, V ;
NEWTON, C ;
WINSTANLEY, P ;
WARN, P ;
PESHU, N ;
PASVOL, G ;
SNOW, R .
NEW ENGLAND JOURNAL OF MEDICINE, 1995, 332 (21) :1399-1404
[7]   Plasmodium falciparum:: effect of anti-malarial drugs on the production and secretion characteristics of histidine-rich protein II [J].
Noedl, H ;
Wongsrichanalai, C ;
Miller, RS ;
Myint, KSA ;
Looareesuwan, S ;
Sukthana, Y ;
Wongchotigul, V ;
Kollaritsch, H ;
Wiedermann, G ;
Wernsdorfer, WH .
EXPERIMENTAL PARASITOLOGY, 2002, 102 (3-4) :157-163
[8]   IDENTIFICATION OF PLASMODIUM-FALCIPARUM HISTIDINE-RICH PROTEIN-2 IN THE PLASMA OF HUMANS WITH MALARIA [J].
PARRA, ME ;
EVANS, CB ;
TAYLOR, DW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (08) :1629-1634
[9]   DETECTION OF ANTIGENS AND ANTIBODIES IN THE URINE OF HUMANS WITH PLASMODIUM-FALCIPARUM MALARIA [J].
RODRIGUEZDELVALLE, M ;
QUAKYI, IA ;
AMUESI, J ;
QUAYE, JT ;
NKRUMAH, FK ;
TAYLOR, DW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (06) :1236-1242
[10]   A quantitative analysis of the microvascular sequestration of malaria parasites in the human brain [J].
Silamut, K ;
Phu, NH ;
Whitty, C ;
Turner, GDH ;
Louwrier, K ;
Mai, NTH ;
Simpson, JA ;
Hien, TT ;
White, NJ .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (02) :395-410