Plasmodium falciparum;
histidine-rich protein 2;
staging;
stage-dependent production;
parasite biomass;
D O I:
10.1016/j.trstmh.2004.11.014
中图分类号:
R1 [预防医学、卫生学];
学科分类号:
1004 ;
120402 ;
摘要:
Because of their sequestration in the microcirculation, the pathogenic late stages of Plasmodium falciparum are under-represented in peripheral blood samples from patients with falciparum malaria. Excreted products of the parasite might help to estimate this sequestered biomass. We quantified the stage-dependent production and release per parasite of P falciparum histidine-rich protein 2 (PfHRP2) with the objective of measuring the sequestered biomass. A simple method to relate parasite stage to parasite age was developed to facilitate this. In four isolates of P falciparum, the median (range) PfHRP2 content was 2.0 fg (0.5-4.3fg) for a young ring stage infected erythrocyte, and 5.4 fg (2.1-10.2 fg) for the schizont stage. The amount of PfHRP2 in the parasitized erythrocyte increased most during development to the mature trophozoite stage. The median (range) amount of PfHRP2 secreted per parasite per entire erythrocytic cycle was 5.2 fg (1.1-13.0 fg). A median of 89% of the total PfHRP2 was excreted at the moment of schizont rupture. This assessment of the stage-dependent release of PfHRP2 is an essential prerequisite for future studies aimed at estimating the total patient parasite mass from the peripheral blood PfHRP2 concentration. (c) 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Choi, CYH
;
Cerda, JF
论文数: 0引用数: 0
h-index: 0
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Cerda, JF
;
Chu, HA
论文数: 0引用数: 0
h-index: 0
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Chu, HA
;
Babcock, GT
论文数: 0引用数: 0
h-index: 0
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Babcock, GT
;
Marletta, MA
论文数: 0引用数: 0
h-index: 0
机构:
Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USAUniv Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Choi, CYH
;
Cerda, JF
论文数: 0引用数: 0
h-index: 0
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Cerda, JF
;
Chu, HA
论文数: 0引用数: 0
h-index: 0
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Chu, HA
;
Babcock, GT
论文数: 0引用数: 0
h-index: 0
机构:Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA
Babcock, GT
;
Marletta, MA
论文数: 0引用数: 0
h-index: 0
机构:
Univ Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USAUniv Michigan, Sch Med, Dept Biol Chem, Div Med Chem, Ann Arbor, MI 48109 USA