Transcriptional analysis and regulation of the sigma-E gene of Streptomyces antibioticus

被引：2

作者：

Bralley, P ^{[1
]}

Jones, GH ^{[1
]}

机构：

[1] Emory Univ, Rollins Res Ctr, Dept Biol, Atlanta, GA 30322 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1517卷 / 03期

关键词：

ECF sigma factor; antibiotic biosynthesis; actinomycin; RNA polymerase;

D O I：

10.1016/S0167-4781(00)00257-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the mapping of the transcriptional start point and identification of the promoter for the sigE gene of Streptomyces antibioticus. Sequence analysis revealed a conserved genetic organization of five genes encompassing sigE in S, antibioticus and S, coelicolor. Upstream of sigE a number of direct repeats, while conserved in both species, are arranged differently. Gel shift analysis demonstrated binding of a component of both S. antibioticus and S. coelicolor crude protein extracts to a 30 bp sequence encompassing one repeat, the A-rich box. Deletion analysis in promoter probes showed that maximal activity of the S. antibioticus promoter depends upon the presence of the sequence surrounding the A-rich box, as well as the region further upstream carrying other direct repeats. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：410 / 415

页数：6

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