Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus

被引：658

作者：

Weigel, LM ^{[1
]}

Clewell, DB

Gill, SR

Clark, NC

McDougal, LK

Flannagan, SE

Kolonay, JF

Shetty, J

Killgore, GE

Tenover, FC

机构：

[1] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA

[2] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA

[3] Inst Genom Res, Rockville, MD 20850 USA

[4] J Craig Center Sci Fdn Joint Technol Ctr, Rockville, MD 20850 USA

来源：

SCIENCE | 2003年 / 302卷 / 5650期

关键词：

D O I：

10.1126/science.1090956

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vancomycin is usually reserved for treatment of serious infections, including those caused by multidrug-resistant Staphylococcus aureus. A clinical isolate of S. aureus with high-level resistance to vancomycin ( minimal inhibitory concentration = 1024 mug/ml) was isolated in June 2002. This isolate harbored a 57.9-kilobase multiresistance conjugative plasmid within which Tn1546 (vanA) was integrated. Additional elements on the plasmid encoded resistance to trimethoprim (dfrA), beta-lactams (blaZ), aminoglycosides (aacA-aphD), and disinfectants ( qacC). Genetic analyses suggest that the long-anticipated transfer of vancomycin resistance to a methicillin-resistant S. aureus occurred in vivo by interspecies transfer of Tn1546 from a co-isolate of Enterococcus faecalis.

引用

页码：1569 / 1571

页数：3

共 21 条

[1]

[Anonymous], 2002, MMWR MORB MORTAL WKL, V51, P902

[2] CHARACTERIZATION OF TN1546, A TN3-RELATED TRANSPOSON CONFERRING GLYCOPEPTIDE RESISTANCE BY SYNTHESIS OF DEPSIPEPTIDE PEPTIDOGLYCAN PRECURSORS IN ENTEROCOCCUS-FAECIUM BM4147 [J].

ARTHUR, M ;

MOLINAS, C ;

DEPARDIEU, F ;

COURVALIN, P .

JOURNAL OF BACTERIOLOGY, 1993, 175 (01) :117-127

[3] Complete nucleotide sequence of pSK41: Evolution of staphylococcal conjugative multiresistance plasmids [J].

Berg, T ;

Firth, N ;

Apisiridej, S ;

Hettiaratchi, A ;

Leelaporn, A ;

Skurray, RA .

JOURNAL OF BACTERIOLOGY, 1998, 180 (17) :4350-4359

[4] MOLECULAR-BASIS FOR VANCOMYCIN RESISTANCE IN ENTEROCOCCUS-FAECIUM BM4147 - BIOSYNTHESIS OF A DEPSIPEPTIDE PEPTIDOGLYCAN PRECURSOR BY VANCOMYCIN RESISTANCE PROTEINS VANH AND VANA [J].

BUGG, TDH ;

WRIGHT, GD ;

DUTKAMALEN, S ;

ARTHUR, M ;

COURVALIN, P ;

WALSH, CT .

BIOCHEMISTRY, 1991, 30 (43) :10408-10415

[5] MOLECULAR ANALYSIS OF A GENTAMICIN RESISTANCE TRANSPOSON-LIKE ELEMENT ON PLASMIDS ISOLATED FROM NORTH-AMERICAN STAPHYLOCOCCUS-AUREUS STRAINS [J].

BYRNE, ME ;

GILLESPIE, MT ;

SKURRAY, RA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (11) :2106-2113

[6] Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene [J].

Chang, S ;

Sievert, DM ;

Hageman, JC ;

Boulton, ML ;

Tenover, FC ;

Downes, FP ;

Shah, S ;

Rudrik, JT ;

Pupp, GR ;

Brown, WJ ;

Cardo, D ;

Fridkin, SK .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (14) :1342-1347

[7] CHARACTERIZATION OF GLYCOPEPTIDE-RESISTANT ENTEROCOCCI FROM UNITED-STATES HOSPITALS [J].

CLARK, NC ;

COOKSEY, RC ;

HILL, BC ;

SWENSON, JM ;

TENOVER, FC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (11) :2311-2317

[8] Contribution of a thickened cell wall and its glutamine nonamidated component to the vancomycin resistance expressed by Staphylococcus aureus Mu50 [J].

Cui, LZ ;

Murakami, H ;

Kuwahara-Arai, K ;

Hanaki, H ;

Hiramatsu, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (09) :2276-2285

[9] DETECTION OF GLYCOPEPTIDE RESISTANCE GENOTYPES AND IDENTIFICATION TO THE SPECIES LEVEL OF CLINICALLY RELEVANT ENTEROCOCCI BY PCR [J].

DUTKAMALEN, S ;

EVERS, S ;

COURVALIN, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :24-27

[10]

Firth N, 2000, GRAM-POSITIVE PATHOGENS, P326

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