Attomole detection of hemagglutinin molecule of influenza virus by combining an electrochemiluminescence sensor with an immunoliposome that encapsulates a Ru complex

被引：55

作者：

Egashira, Naoyoshi ^{[1
]}

Morita, Shin-ichi ^{[1
]}

Hifumi, Emi ^{[2
,3
]}

Mitoma, Yoshiharu ^{[1
]}

Uda, Taizo ^{[3
,4
]}

机构：

[1] Prefectural Univ Hiroshima, Dept Environm Sci, Fac Life & Environm Sci, Shobara Shi, Hiroshima, Japan

[2] Oita Univ, Res Ctr Appl Med Engn, Oita 8701192, Japan

[3] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

[4] Oita Univ, Fac Engn, Dept Appl Chem, Oita 8701192, Japan

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 11期

关键词：

D O I：

10.1021/ac702625d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immunoliposome (80 nm in diameter) encapsulating a Ru complex with two aminobutyl moieties was prepared to detect the presence of hemagglutinin molecules, which play an important role in influenza virus infection. The highly sensitive detection was accomplished by electro-chemiluminescence (ECL) from the Ru complex adsorbed onto Au electrodes after competitive immunoreactions. This method clarified that the adsorption of the Ru complex onto the electrode was an important factor in obtaining high sensitivity. Optimization of the analytical conditions enabled determination of the hemagglutinin molecules of the influenza virus in the concentration range of 3 x 10(-14) (6 x 10(-19) mol/50 mu L sample) to 2 x 10(-12) g/mL. The sensitivity was far superior to that obtained by conventional ELISA as well as to that obtained by biosensors and reported thus far.

引用

页码：4020 / 4025

页数：6

共 18 条

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