Models to elucidate the regulation of adrenal cell death

被引:11
作者
Carsia, RV [1 ]
Nagele, RG
Morita, Y
Tilly, KI
Tilly, JL
机构
[1] Univ Med & Dent New Jersey, Sch Osteopath Med, Dept Cell Biol, Stratford, NJ 08084 USA
[2] Univ Med & Dent New Jersey, Sch Osteopath Med, Dept Mol Biol, Stratford, NJ 08084 USA
[3] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Vincent Ctr Reprod Biol,Dept Obstet & Gynecol, Boston, MA USA
关键词
D O I
10.3109/07435809809032705
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Hypophysectomy-induced apoptosis in the rat adrenal gland is slow (not apparent until 12-24 h) and in-situ, 3'-end labeling (ISEL) of DNA strand breaks is restricted to a subpopulation of zona reticularis cells. In addition, it is completely blocked by ACTH. By contrast, apoptosis in the intact rat adrenal gland, cultured in the absence of trophic support, is extensive and rapid. Culture-triggered apoptosis las determined by oligonucleosome formation) is attenuated by ACTH and is largely restricted to the zonae fasciculata and reticularis even at 3 h las determined by ISEL and DAPI cytochemistry). Thus, this organ culture system may help elucidate factors that can acutely regulate adrenocortical cell survival. Quartered glands have a nearly 2-fold increase in oligonucleosome formation compared to intact glands at 3 h and are resistant to the antiapoptotic action of ACTH. In contrast, ACTH-induced corticosterone secretion is not attenuated. Angiotensin II (Ang II) enhances culture-triggered apoptosis, and its apoptotic action is attenuated by ACTH. These observations suggest that 1) acute hormonal modulation of apoptosis may require some level of gross adrenal structural integrity, and 2) ACTH and Ang II act in an antagonistic fashion to regulate adrenocortical apoptosis. The apoptotic effect of Ang II may be mediated via the type 2 receptor.
引用
收藏
页码:899 / 908
页数:10
相关论文
共 21 条
  • [1] ARENDS MJ, 1990, AM J PATHOL, V136, P593
  • [2] Apoptotic cell death in the rat adrenal gland: An in vivo and in vitro investigation
    Carsia, RV
    Macdonald, GJ
    Gibney, JA
    Tilly, KI
    [J]. CELL AND TISSUE RESEARCH, 1996, 283 (02) : 247 - 254
  • [3] Hormonal modulation of apoptosis in the rat adrenal gland in vitro is dependent on structural integrity
    Carsia, RV
    Tilly, KI
    Tilly, JL
    [J]. ENDOCRINE, 1997, 7 (03) : 377 - 381
  • [4] CARSIA RV, 1997, END SOC 79 ANN M, P206
  • [5] ADRENOCORTICAL APOPTOSIS IN HYPOPHYSECTOMIZED RATS IS SELECTIVELY REDUCED BY ACTH
    CECCATELLI, S
    DIANA, A
    VILLAR, MJ
    NICOTERA, P
    [J]. NEUROREPORT, 1995, 6 (02) : 342 - 344
  • [6] Expression of p21(WAF1/CIP1/SDI1) and p53 in apoptotic cells in the adrenal cortex and induction by ischemia reperfusion injury
    Didenko, VV
    Wang, XD
    Yang, LQ
    Hornsby, PJ
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (07) : 1723 - 1731
  • [7] IN-SITU DETECTION OF FRAGMENTED DNA (TUNEL ASSAY) FAILS TO DISCRIMINATE AMONG APOPTOSIS, NECROSIS, AND AUTOLYTIC CELL-DEATH - A CAUTIONARY NOTE
    GRASLKRAUPP, B
    RUTTKAYNEDECKY, B
    KOUDELKA, H
    BUKOWSKA, K
    BURSCH, W
    SCHULTEHERMANN, R
    [J]. HEPATOLOGY, 1995, 21 (05) : 1465 - 1468
  • [8] APOPTOSIS - BASIC BIOLOGICAL PHENOMENON WITH WIDE-RANGING IMPLICATIONS IN TISSUE KINETICS
    KERR, JFR
    WYLLIE, AH
    CURRIE, AR
    [J]. BRITISH JOURNAL OF CANCER, 1972, 26 (04) : 239 - +
  • [9] SHRINKAGE NECROSIS OF ADRENAL CORTICAL CELLS
    KERR, JFR
    [J]. JOURNAL OF PATHOLOGY, 1972, 107 (03) : 217 - +
  • [10] Krajewski S, 1996, CANCER RES, V56, P2849