Engineered Anopheles Immunity to Plasmodium Infection

被引：136

作者：

Dong, Yuemei ^{[1
]}

Das, Suchismita ^{[1
]}

Cirimotich, Chris ^{[1
]}

Souza-Neto, Jayme A. ^{[1
]}

McLean, Kyle J. ^{[1
]}

Dimopoulos, George ^{[1
]}

机构：

[1] Johns Hopkins Univ, W Harry Feinstone Dept Mol Microbiol & Immunol, Bloomberg Sch Publ Hlth, Baltimore, MD 21218 USA

来源：

PLOS PATHOGENS | 2011年 / 7卷 / 12期

基金：

美国国家卫生研究院;

关键词：

MALARIA PARASITE; DROSOPHILA-MELANOGASTER; VITELLOGENIN GENE; INNATE IMMUNITY; LIFE-CYCLE; MOSQUITO; GAMBIAE; VECTOR; STEPHENSI; TRANSMISSION;

D O I：

10.1371/journal.ppat.1002458

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A causative agent of human malaria, Plasmodium falciparum, is transmitted by Anopheles mosquitoes. The malaria parasite is under intensive attack from the mosquito's innate immune system during its sporogonic development. We have used genetic engineering to create immune-enhanced Anopheles stephensi mosquitoes through blood meal-inducible expression of a transgene encoding the IMD pathway-controlled NF-kB Rel2 transcription factor in the midgut and fat-body tissue. Transgenic mosquitoes showed greater resistance to Plasmodium and microbial infection as a result of timely concerted tissue-specific immune attacks involving multiple effectors. The relatively weak impact of this genetic modification on mosquito fitness under laboratory conditions encourages further investigation of this approach for malaria control.

引用

页数：12

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