Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex

被引：129

作者：

Gong, LM ^{[1
]}

Li, B ^{[1
]}

Millas, S ^{[1
]}

Yeh, ETH ^{[1
]}

机构：

[1] Univ Texas, Hlth Sci Ctr, Inst Mol Med Prevent Human Dis, Res Ctr Cardiovasc Dis, Houston, TX 77030 USA

来源：

FEBS LETTERS | 1999年 / 448卷 / 01期

关键词：

ubiquitin; sentrin; SUMO; UBC9; UBA2; AOS1;

D O I：

10.1016/S0014-5793(99)00367-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a beta-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins. (C) 1999 Federation of European Biochemical Societies.

引用

页码：185 / 189

页数：5

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