Application of bovine pituitary extract and polyglycolide/poly(lactide-co-glycolide) scaffold to the cultivation of bovine knee chondrocytes

被引：20

作者：

Kuo, YC ^{[1
]}

Chung, CY ^{[1
]}

机构：

[1] Natl Chung Cheng Univ, Dept Chem Engn, Chiayi 62102, Taiwan

来源：

BIOTECHNOLOGY PROGRESS | 2005年 / 21卷 / 06期

关键词：

D O I：

10.1021/bp050234s

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In vitro cultivation of primary bovine knee chondrocytes (BKCs), using bovine pituitary extract (BPE) and porous scaffolds composed of polyglycolide (PGA) and 85/15 poly(lactide-co-glycolide) (PLGA), was investigated. Here, BPE was prepared from fresh bovine pituitaries, and cylindrical PGA/PLGA scaffolds with various chemical compositions were fabricated by solvent merging/particulate leaching method. Experimental results showed that in microcarrier systems, the rate of BKC growth on PGA surfaces is faster than that on PLGA surfaces, and the decrease in the medium pH value of BKCs-adsorbed PGA particles is faster than that of BKCs-adsorbed PLGA particles. After 28-day construct cultivation, the BKC amount and the content of glycosamino-glycans and collagen per construct increased with BPE protein concentration. For a constant BPE protein concentration, a higher PGA percentage in scaffold leads to a better biological environment for the growth of BKCs and the synthesis of extracellar matrices.

引用

页码：1708 / 1715

页数：8

共 46 条

[1]

Andriano KP, 1999, J BIOMED MATER RES, V48, P602, DOI 10.1002/(SICI)1097-4636(1999)48:5<602::AID-JBM3>3.0.CO

[2]

2-6

[3] IDENTIFICATION OF TRYPSIN-INHIBITOR IN BOVINE PITUITARY EXTRACTS AS A SURVIVAL FACTOR FOR ADULT-RAT HEPATOCYTES IN PRIMARY CULTURE [J].

ASAMI, O ;

NAKAMURA, T ;

MURA, T ;

ICHIHARA, A .

JOURNAL OF BIOCHEMISTRY, 1984, 95 (02) :299-309

[4]

BELL JA, 1985, J BIOL CHEM, V260, P8520

[5]

BERGMAN M, 1961, ANAL BIOCHEM, V25, P1961

[6]

Boyce S.T., 1985, J TISS CULT METH, V9, P83, DOI DOI 10.1007/BF01797779

[7] Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels [J].

Chaipinyo, K ;

Oakes, BW ;

van Damme, MPI .

JOURNAL OF ORTHOPAEDIC RESEARCH, 2002, 20 (05) :1070-1078

[8] Biomimetic surface modification of poly(L-lactic acid) with chitosan and its effects on articular chondrocytes in vitro [J].

Cui, YL ;

Di Qi, A ;

Liu, WG ;

Wang, XH ;

Wang, H ;

Ma, DM ;

De Yao, K .

BIOMATERIALS, 2003, 24 (21) :3859-3868

[9] Controlled-release of IGF-I and TGF-β1 in a photopolymerizing hydrogel for cartilage tissue engineering [J].

Elisseeff, J ;

McIntosh, W ;

Fu, K ;

Blunk, T ;

Langer, R .

JOURNAL OF ORTHOPAEDIC RESEARCH, 2001, 19 (06) :1098-1104

[10] Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue [J].

Enobakhare, BO ;

Bader, DL ;

Lee, DA .

ANALYTICAL BIOCHEMISTRY, 1996, 243 (01) :189-191

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