Determination of the alkaloids in evodiae fructus by high-performance liquid chromatography

被引：28

作者：

Chuang, WC ^{[1
]}

Chu, CY ^{[1
]}

Sheu, SJ ^{[1
]}

机构：

[1] NATL TAIWAN NORMAL UNIV,DEPT CHEM,TAIPEI,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 727卷 / 02期

关键词：

Evodiae Fructus; alkaloids;

D O I：

10.1016/0021-9673(95)01220-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the simultaneous determination of four indolequinazoline alkaloids (carboxyevodiamine, dehydroevodiamine, evodiamine and rutaecarpine) and eight quinolone alkaloids (1-methyl-2-nonyl-4 (1H)-quinolone, 1-methyl-2-[(Z)-6-undecenyl]-4(1H)-quinolone, 1-methyl-2-undecyl-4(1H)-quinolone, evocarpine, 1-methyl-2-[(6Z,9Z)-6,9-pentadecadienyl]-4(1H)-quinolone, dihydroevocarpine, 1-methyl-2-[(Z)-10-pentadecenyl]-4(1H)-quinolone and 1-methyl-2-[(Z)-6-pentadecenyl]-4(1H)-quinolone) in Evodiae Fructus was developed. The method was carried out by using a Capcell C-18 SG-120 column with a gradient solvent system of acetate buffer-acetonitrile-methanol and a UV detector (250 nm), and the contents of the alkaloids in a non-pretreated Evodiae Fructus extract could easily be determined within 40 min. Validation of this method is described.

引用

页码：317 / 323

页数：7

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