Anti-invasive activity of diallyl disulfide through tightening of tight junctions and inhibition of matrix metalloproteinase activities in LNCaP prostate cancer cells

被引：43

作者：

Shin, Dong Yeok ^{[3
,4
]}

Kim, Gi-Young ^{[5
]}

Kim, Jung-In ^{[6
]}

Yoon, Moo Kyoung ^{[7
]}

Kwon, Taeg Kyu ^{[8
]}

Lee, Su Jae ^{[9
]}

Choi, Young-Whan ^{[10
]}

Kang, Ho Sung ^{[11
]}

Yoo, Young Hyun ^{[1
,2
]}

Choi, Yung Hyun ^{[3
,4
,12
]}

机构：

[1] Dong A Univ, Coll Medicine, Dept Anat & Cell Biol, Pusan 602714, South Korea

[2] Mitochondria Hub Regulat Ctr, Pusan 602714, South Korea

[3] Dong Eui Univ, Coll Oriental Med, Dept Biochem, Pusan 614714, South Korea

[4] Dong Eui Univ, Coll Oriental Med, Res Inst Oriental Med, Pusan 614714, South Korea

[5] Jeju Natl Univ, Dept Marine Life Sci, Cheju 690756, South Korea

[6] Inje Univ, Sch Food & Life Sci, Kimhae 621749, South Korea

[7] Natl Inst Hort & Herbal Sci, Vegetable Res Div, Suwon 440706, South Korea

[8] Keimyung Univ, Sch Med, Dept Immunol, Taegu 704701, South Korea

[9] Hanyang Univ, Res Inst Nat Sci, Dept Chem, Seoul 133791, South Korea

[10] Pusan Natl Univ, Coll Nat Resource & Life Sci, Dept Hort Biosci, Miryang 627706, South Korea

[11] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

[12] Dong Eui Univ, Reg Innovat Ctr, Grad Sch & Blue Bio Ind, Dept Biomat Control, Pusan 614714, South Korea

来源：

TOXICOLOGY IN VITRO | 2010年 / 24卷 / 06期

关键词：

DADS; Invasion; Tight junctions; Matrix metalloproteinases; NF-KAPPA-B; HISTONE ACETYLATION; BETA-CATENIN; EXPRESSION; CARCINOMA; APOPTOSIS; CLAUDIN-4; CADHERIN; INDUCTION; PROTEINS;

D O I：

10.1016/j.tiv.2010.06.014

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

Diallyl disulfide (DADS) is a major component of an oil-soluble allyl sulfide garlic (Allium sativum) derivative, which has been shown to exert a potential for anti-cancer activity. However, the biochemical mechanisms underlying DADS-induced anti-invasiveness and anti-metastasis have not been thoroughly studied. In this study, we investigated the effect of DADS on the correlation between tightening of tight junctions (TJs) and anti-invasive activity in human prostate carcinoma LNCaP cells. Inhibitory effects DADS on cell motility and invasiveness were found to be associated with increased tightness of the TJ, which was demonstrated by an increase in transepithelial electrical resistance (TER). Additionally, immunoblotting results indicated that DADS repressed the levels of the claudin proteins, which are major components of TJs that play a key role in control and selectivity of paracellular transport. Furthermore, the activities of matrix metalloproteinase (MMP)-2 and -9 in LNCaP cells were dose-dependently inhibited by treatment with DADS, and this was also correlated with a decrease in expression of their mRNA and proteins. Although further studies are needed, the present study indicates that TJs and MMPs are critical targets of DADS-induced anti-invasiveness in human prostate cancer LNCaP cells. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1569 / 1576

页数：8

共 42 条

[1]

Claudin-3 and claudin-4 expression in ovarian epithelial cells enhances invasion and is associated with increased matrix metalloproteinase-2 activity [J].