Zerumbone induces apoptosis in T-acute lymphoblastic leukemia cells

被引：51

作者：

Abdelwahab, Siddig Ibrahim ^{[1
]}

Abdul, Ahmad Bustamam ^{[1
]}

Mohan, Syam ^{[1
]}

Taha, Manal Mohamed Elhassan ^{[1
]}

Syam, Suvitha ^{[1
]}

Ibrahim, Mohamed Yousif ^{[1
]}

Mariod, Abdelbasit Adam ^{[1
]}

机构：

[1] Univ Putra Malaysia, Inst Biosci, UPM MAKNA Canc Res Lab, Serdang 43400, Malaysia

来源：

LEUKEMIA RESEARCH | 2011年 / 35卷 / 02期

关键词：

Apoptosis; Scanning electron microscope; Zerumbone; TUNEL assay; SESQUITERPENE;

D O I：

10.1016/j.leukres.2010.07.025

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Zerumbone (ZER) is a potential anticancer natural compound, isolated from Zingiber zerumbet Smith. In this investigation, the anticancer properties of ZER were evaluated on cancer cells of T-acute lymphoblastic leukemia, CEM-ss. The results showed that ZER has cytotoxic effect against CEM-ss cells with an IC50 of 8.4 +/- 1.9 mu g/ml (coefficient of variation < 30%). Comparatively, 5-fluorouracil (positive control), imposed an inhibitory effect on CEM-ss cells with an IC50 of 1.94 +/- 0.06 mu g/ml. Scanning electron microscopy (SEM) results revealed abnormalities such as membrane blebbing, holes and cytoplasmic extrusions, all of which are characteristics of apoptosis. In addition, ZER has increased the number of TUNEL-positive stain and the cellular level of caspase-3, the hallmarks of apoptosis, on treated CEM-ss cells. It could be concluded that, ZER was able to produce apoptosis on T-acute lymphoblastic leukemia, CEM-ss. The current findings suggest that ZER might be helpful for improving the usefulness of anticancer agents in the therapy of leukemia. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：268 / 271

页数：4

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