A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

被引：76

作者：

Sedlak, Ruth Hall ^{[1
]}

Kuypers, Jane ^{[1
]}

Jerome, Keith R. ^{[1
,2
]}

机构：

[1] Univ Washington, Dept Lab Med, Mol Virol Lab, Seattle, WA 98195 USA

[2] Fred Hutchinson Canc Res Ctr, Vaccine Infect Dis Div, Seattle, WA 98104 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 80卷 / 04期

关键词：

Digital PCR; Inhibition; Quantitative PCR; Stool; CYTOMEGALOVIRUS DNA;

D O I：

10.1016/j.diagmicrobio.2014.09.004

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：285 / 286

页数：2

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