Using fluorescence lifetime for discriminating detector afterpulsing in fluorescence-correlation spectroscopy

被引：93

作者：

Enderlein, J ^{[1
]}

Gregor, I ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Biol Informat Proc 1 & 2, D-52425 Julich, Germany

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2005年 / 76卷 / 03期

关键词：

D O I：

10.1063/1.1863399

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Fluorescence correlation spectroscopy (FCS) has become an important and widely used technique for many applications in physics, chemistry, and biology. Usually, FCS is measured with sensitive light detectors working in the photon-counting Geiger mode. A common property of such detectors is afterpulsing: the generation of spurious photon detection events after a genuine photon detection. Such afterpulsing causes a significant deviation of the measured autocorrelation function from its true value on a short time scale and can seriously influence derived parameters for fast processes such as triplet-state photophysics. Here, we discuss the impact of afterpulsing on FCS in detail. A new method is developed to eliminate afterpulsing effects by using time-correlated single-photon counting for separating the true fluorescence signal from afterpulsing events. (C) 2005 American Institute of Physics.

引用

页数：5

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