Aspirin dose dependently inhibits the interleukin-1 β-stimulated increase in inducible nitric oxide synthase, nitric oxide, and prostaglandin E2 production in rat ovarian dispersates cultured in vitro

Objective: Determine if aspirin inhibits the IL-1 beta -stimulated expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and prostaglandin E-2 (PGE(2)) in mt ovarian dispersates cultured in vitro. Design: Prospective, controlled in vitro study. Setting: Academic research laboratory. animals: Ovaries collected from immature rats. Intervention(s): Ovaries were collected from immature mts and enzymatically dispersed. Ovarian dispersates were placed into plates containing media alone or media supplemented with IL-1 beta (100 U/mL) and varying concentrations of aspirin (0, 1, 3, 5 and 10 mM). Ovarian dispersates were cultured in a environment of 5% CO2 in air at 37 degreesC for 24 or 38 hours. Main Outcome Measure(s): Twenty-four- and 48-hour iNOS, nitrite (a stable metabolite of NO), and PGE, levels were determined from ovarian dispersates cultured in vitro. Result(s): Administration of IL-1 beta increased nitrite and PGE(2) levels over that observed in the control group after cuture of ovarian dispersates for 24 and 48 hours. Aspirin dose dependently reduced the IL-1 beta pstimulated increase in nitrite production from ovarian dispersates after culture for 24 and 48 hours. Aspirin completely (24 hours) or dose dependently (48 hours) prevented the IL-beta -stimulnted increase in PGE(2) Coadministration of IL-1 beta and aspirin (10 mM) attenuates IL-1 beta -stimulated iNOS expression after culture for 24 and 48 hours. Conclusion(s): Aspirin significantly inhibits the IL-1 beta -stimulated expression of iNOS, NO, and PGE, in ovarian dispersates cultured in vitro. (Fertil Steril (R) 2001;75:778-84. (C) 2001 by American Society for Reproductive Medicine.)