A very stable cyclic DNA miniduplex with just two base pairs

被引：50

作者：

El-Sagheer, Afaf H. ^{[1
]}

Kumar, Ravindra ^{[1
]}

Findlow, Stuart ^{[2
]}

Werner, Joern M. ^{[2
]}

Lane, Andrew N. ^{[3
]}

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England

[3] Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40202 USA

来源：

CHEMBIOCHEM | 2008年 / 9卷 / 01期

关键词：

cyclic; DNA recognition; DNA structure; DNA; miniduplex;

D O I：

10.1002/cbic.200700538

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) What goes around... Cyclic mini-DNA duplexes, as shown in the scheme, have been synthesized in high yield on the multimicromole scale by using click chemistry. These B-like duplexes are very stable and bind to drugs, such as distamycin A and 7-aminoactinomycin D. Circular dichroism indicates that the bases are stacked, and UV melting and NMR spectroscopy showed that the interbase hydrogen bonds are very stable, even in a dimer. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：50 / 52

页数：3

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