The DNA binding of insect Fork head factors is strongly influenced by the negative cooperation of neighbouring bases

被引:14
作者
Takiya, S
Gazi, M
Mach, V [1 ]
机构
[1] Hokkaido Univ, Grad Sch Environm Earth Sci, Ctr Adv Sci & Technol, Kita Ku, Sapporo, Hokkaido 0600810, Japan
[2] Hokkaido Univ, Grad Sch Environm Earth Sci, Div Biosci, Kita Ku, Sapporo, Hokkaido 0600810, Japan
[3] Univ S Bohemia, Fac Sci, Ceske Budejovice, Czech Republic
[4] Acad Sci Czech Republ, Inst Entomol, Ceske Budejovice, Czech Republic
关键词
drosophila; bombyx; galleria; positioned binding site; dinucleotide step; transcription; promoter;
D O I
10.1016/s0965-1748(03)00134-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Drosophila melanogaster Fork head and Bombyx mori SGF1/Fork head proteins are key regulators of tissue specific gene expression in the modified larval labial glands. Here we use the competitive electrophoretic mobility shift assay to create a detailed Fork head binding matrix and we investigate some unusual features of the Fork head interaction with DNA. We found that the Fork head-DNA interaction is context dependent-the binding specificity of the protein is partly determined by specific combinations of neighbouring bases. Although the total number of the sub-optimal dinucleotide steps is not high, the negative cooperation of neighbouring bases significantly contributes to the overall binding site specificity. Our results allow efficient recognition of insect Fork head binding sites and we show that the putative Fork head cognate elements preferentially accumulate in the near upstream region of genes abundantly expressed in the labial gland. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1145 / 1154
页数:10
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