Interferon-γ stimulates the expression of CX3CL1/fractalkine in cultured human endothelial cells

被引：52

作者：

Imaizumi, T

Matsumiya, T

Fujimoto, K

Okamoto, K

Cui, XF

Ohtaki, U

Yoshida, H

Satoh, K

机构：

[1] Hirosaki Univ, Sch Med, Inst Brain Sci, Dept Vasc Surg, Hirosaki, Aomori 0368562, Japan

[2] Hirosaki Univ, Sch Med, Dept Dent & Oral Surg, Hirosaki, Aomori 0368562, Japan

来源：

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE | 2000年 / 192卷 / 02期

关键词：

fractalkine; endothelial cells; interferon-gamma;

D O I：

10.1620/tjem.192.127

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

CX3CL1/Fractalkine, a CX3C chemokine, is a potent agonist for the chemotaxis and adhesion of monocytes and lymphocytes. It was first identified as a membrane protein in endothelial cells activated with IL-1 or TNF-alpha. We have found the enhanced expression of fractalkine in human umbilical vein endothelial cells stimulated with interferon-gamma (IFN-gamma). Pretreatment of the cells with cycloheximide did not inhibit the expression of fractalkine mRNA. The majority of fractalkine protein was found in the cell lysate, and an antibody-blocking experiment disclosed that fractalkine contributes to the adhesion of mononuclear cells to endothelial monolayers stimulated with IFN-gamma. Vascular endothelial cells produce fractalkine in response to IFN-gamma, and this may play an important role in immune responses by eliciting a traffic of mononuclear cells through the vascular wall. (C) 2000 Tohoku University Medical Press.

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收藏

页码：127 / 139

页数：13

相关论文

共 30 条

[1] A new class of membrane-bound chemokine with a CX(3)C motif [J].

Bazan, JF ;

Bacon, KB ;

Hardiman, G ;

Wang, W ;

Soo, K ;

Rossi, D ;

Greaves, DR ;

Zlotnik, A ;

Schall, TJ .

NATURE, 1997, 385 (6617) :640-644

[2]

BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P77

[3] TRANSCRIPTIONAL REGULATION OF ENDOTHELIAL-CELL ADHESION MOLECULES - NF-KAPPA-B AND CYTOKINE-INDUCIBLE ENHANCERS [J].

COLLINS, T ;

READ, MA ;

NEISH, AS ;

WHITLEY, MZ ;

THANOS, D ;

MANIATIS, T .

FASEB JOURNAL, 1995, 9 (10) :899-909

[4] Fractalkine and CX3CR1 mediate a novel mechanism of leukocyte capture, firm adhesion, and activation under physiologic flow [J].

Fong, AM ;

Robinson, LA ;

Steeber, DA ;

Tedder, TF ;

Yoshie, O ;

Imai, T ;

Patel, DD .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (08) :1413-1419

[5] NF-κB-dependent fractalkine induction in rat aortic endothelial cells stimulated by IL-1β, TNF-α, and LPS [J].

Garcia, GE ;

Xia, YY ;

Chen, SZ ;

Wang, YB ;

Ye, RD ;

Harrison, JK ;

Bacon, KB ;

Zerwes, HG ;

Feng, LL .

JOURNAL OF LEUKOCYTE BIOLOGY, 2000, 67 (04) :577-584

[6] Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia [J].

Harrison, JK ;

Jiang, Y ;

Chen, SZ ;

Xia, YY ;

Maciejewski, D ;

McNamara, RK ;

Streit, WJ ;

Salafranca, MN ;

Adhikari, S ;

Thompson, DA ;

Botti, P ;

Bacon, KB ;

Feng, LL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10896-10901

[7] Identification and molecular characterization of fractalkine receptor CX(3)CR1, which mediates both leukocyte migration and adhesion [J].

Imai, T ;

Hieshima, K ;

Haskell, C ;

Baba, M ;

Nagira, M ;

Nishimura, M ;

Kakizaki, M ;

Takagi, S ;

Nomiyama, H ;

Schall, TJ ;

Yoshie, O .

CELL, 1997, 91 (04) :521-530

[8]

Imaizumi T, 2000, THROMB HAEMOSTASIS, V83, P949

[9] Expression of tumor necrosis factor-α in cultured human endothelial cells stimulated with lipopolysaccharide or interleukin-1α [J].

Imaizumi, T ;

Itaya, H ;

Fujita, K ;

Kudoh, D ;

Kudoh, S ;

Mori, K ;

Fujimoto, K ;

Matsumiya, T ;

Yoshida, H ;

Satoh, K .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2000, 20 (02) :410-415

[10] Human endothelial cells synthesize ENA-78: Relationship to IL-8 and to signaling of PMN adhesion [J].

Imaizumi, TA ;

Albertine, KH ;

Jicha, DL ;

McIntyre, TM ;

Prescott, SM ;

Zimmerman, GA .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1997, 17 (02) :181-192