Role of nitric oxide in systemic vasopressin-induced hypothermia

被引:52
作者
Steiner, AA
Carnio, EC
Antunes-Rodrigues, J
Branco, LGS [1 ]
机构
[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Fisiol, BR-14040904 Ribeirao Preto, Brazil
[2] Univ Sao Paulo, Escola Enfermagem Ribeirao Preto, BR-14040904 Ribeirao Preto, Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14040904 Ribeirao Preto, Brazil
关键词
endothelium-derived relaxing factor; temperature; nitric oxide synthase; arginine vasopressin;
D O I
10.1152/ajpregu.1998.275.4.R937
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
It has been reported that arginine vasopressin (AVP) plays a thermoregulatory action, but very little is known about the mechanisms involved. In the present study, we tested the hypothesis that nitric oxide (NO) plays a role in systemic AVP-induced hypothermia. Rectal temperature was measured before and after AVP, AVP blocker, or N-G-nitro-L-arginine methyl ester (L-NAME; NO synthase inhibitor) injection. Control animals received saline injections of the same volume. The basal body temperature (Tb) measured in control animals was 36.53 +/- 0.08 degrees C. We observed a significant (P < 0.05) reduction in T-b to 35.44 +/- 0.19 degrees C after intravenous injection of AVP (2 mu g/kg) and to 35.74 +/- 0.10 degrees C after intravenous injection of L-NAME (30 mg/kg). The systemic injection of the AVP blocker [beta-mercapto-beta, beta-cyclopenta-methylenepropionyl(1),O-Et-Tyr(2),Val(4),Arg(8)]vasopressin (10 mu g/kg) caused a significant increase in Tb to 37.33 +/- 0.23 degrees C, indicating that AVP plays a tonic role by reducing Tb. When the treatments with AVP and L-NAME were combined, systemically injected L-NAME blunted AVP-induced hypothermia. To assess the role of central thermoregulatory mechanisms, a smaller dose of L-NAME (1 mg/kg) was injected into the third cerebral ventricle. Intracerebroventricular injection of L-NAME caused an increase in Tb, but when intracerebroventricular L-NAME was combined with systemic AVP injection (2 mu g/kg), no change in Tb was observed. The data indicate that central NO plays a major role mediating systemic AVP-induced hypothermia.
引用
收藏
页码:R937 / R941
页数:5
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