Ultrastructure and immunolabelling of fungi cells treated with Vigna unguiculata vicilins (7S storage proteins)

被引：18

作者：

Gomes, VM

Da Cunha, M

Miguens, FC

Fernandes, KVS

Rose, TL

Xavier, J

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Prot & Peptideos, BR-28015620 Rio De Janeiro, Brazil

[2] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Fisiol & Bioquim Microrganismos, Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestruct Celular Hertha Meyer, BR-21941 Rio De Janeiro, Brazil

来源：

PLANT SCIENCE | 1998年 / 138卷 / 01期

关键词：

vicilin; 7S storage globulins; chitin binding proteins; immunolabelling;

D O I：

10.1016/S0168-9452(98)00148-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Scanning electron microscopy showed that vicilins from cowpea seeds affect the growth and inhibit spore germination of Fusarium oxysporum and leads to abnormal development (sporulation) of Saccharomyces cerevisiae cells. Control treatments induced normal hyphal and cell development. Fluorescence microscopy and immunolabelling analysis showed that anti-vicilin serum strongly labelled the cell surfaces of yeast and filamentous fungi, previously incubated with these seed proteins. These results suggest that the inhibitory effects of cowpea vicilins on fungi growth may be exerted through their ability to bind to the cell surfaces of the micro-organisms, cell wall and/or plasma membrane. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：81 / 89

页数：9

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