Recent advances in awakening silent biosynthetic gene clusters and linking orphan clusters to natural products in microorganisms

被引：157

作者：

Chiang, Yi-Ming ^{[1
,2
]}

Chang, Shu-Lin ^{[1
,3
]}

Oakley, Berl R. ^{[4
]}

Wang, Clay C. C. ^{[1
,5
]}

机构：

[1] Univ So Calif, Dept Pharmacol & Pharmaceut Sci, Sch Pharm, Los Angeles, CA 90089 USA

[2] Chia Nan Univ Pharm & Sci, Grad Inst Pharmaceut Sci, Tainan 71710, Taiwan

[3] Chia Nan Univ Pharm & Sci, Dept Biotechnol, Tainan 71710, Taiwan

[4] Univ Kansas, Dept Mol Biosci, Lawrence, KS 66045 USA

[5] Univ So Calif, Dept Chem, Coll Letters Arts & Sci, Los Angeles, CA 90089 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2011年 / 15卷 / 01期

关键词：

ASPERGILLUS-NIDULANS; POLYKETIDE SYNTHASES; CHEMICAL DIVERSITY; DISCOVERY; STRATEGIES; PATHWAYS; DOMAINS; LOGIC;

D O I：

10.1016/j.cbpa.2010.10.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Secondary metabolites from microorganisms have a broad spectrum of applications, particularly in therapeutics. The growing number of sequenced microbial genomes has revealed a remarkably large number of natural product biosynthetic clusters for which the products are still unknown. These cryptic clusters are potentially a treasure house of medically useful compounds. The recent development of new methodologies has made it possible to begin unlock this treasure house, to discover new natural products and to determine their biosynthesis pathways. This review will highlight some of the most recent strategies to activate silent biosynthetic gene clusters and to elucidate their corresponding products and pathways.

引用

页码：137 / 143

页数：7

共 35 条

[1] Cloning and characterization of new glycopeptide gene clusters found in an environmental DNA megalibrary [J].

Banik, Jacob J. ;

Brady, Sean F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (45) :17273-17277

[2] Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans [J].

Bergmann, Sebastian ;

Schuemann, Julia ;

Scherlach, Kirstin ;

Lange, Corinna ;

Brakhage, Axel A. ;

Hertweck, Christian .

NATURE CHEMICAL BIOLOGY, 2007, 3 (04) :213-217

[3]

Bode HB, 2002, CHEMBIOCHEM, V3, P619, DOI 10.1002/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO

[4]

2-9

[5] Chromatin-level regulation of biosynthetic gene clusters [J].

Bok, Jin Woo ;

Chiang, Yi-Ming ;

Szewczyk, Edyta ;

Reyes-Domingez, Yazmid ;

Davidson, Ashley D. ;

Sanchez, James F. ;

Lo, Hsien-Chun ;

Watanabe, Kenji ;

Strauss, Joseph ;

Oakley, Berl R. ;

Wang, Clay C. C. ;

Keller, Nancy P. .

NATURE CHEMICAL BIOLOGY, 2009, 5 (07) :462-464

[6] Genomic mining for Aspergillus natural products [J].

Bok, JW ;

Hoffmeister, D ;

Maggio-Hall, LA ;

Murillo, R ;

Glasner, JD ;

Keller, NP .

CHEMISTRY & BIOLOGY, 2006, 13 (01) :31-37

[7] Fungal secondary metabolites - Strategies to activate silent gene clusters [J].

Brakhage, Axel A. ;

Schroeckh, Volker .

FUNGAL GENETICS AND BIOLOGY, 2011, 48 (01) :15-22

[8] A proteomics approach to discovering natural products and their biosynthetic pathways [J].

Bumpus, Stefanie B. ;

Evans, Bradley S. ;

Thomas, Paul M. ;

Ntai, Ioanna ;

Kelleher, Neil L. .

NATURE BIOTECHNOLOGY, 2009, 27 (10) :951-U120

[9]

Chiang YM, 2009, NAT PROD COMMUN, V4, P1505

[10] A Gene Cluster Containing Two Fungal Polyketide Synthases Encodes the Biosynthetic Pathway for a Polyketide, Asperfuranone, in Aspergillus nidulans [J].

Chiang, Yi-Ming ;

Szewczyk, Edyta ;

Davidson, Ashley D. ;

Keller, Nancy ;

Oakley, Berl R. ;

Wang, Clay C. C. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (08) :2965-2970

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