Expression, purification, crystallization and crystallographic characterization of the human MHC class I related protein MICA

被引：14

作者：

Bauer, S

Willie, ST

Spies, T

Strong, RK

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA

[2] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1998年 / 54卷

关键词：

D O I：

10.1107/S0907444997015229

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of the human MHC-encoded molecule MICA, a homologue of MHC class I proteins, have been grown in hanging-drop vapor-diffusion trials using ammonium sulfate as a precipitating agent with recombinant protein expressed in a baculovirus-based system. Cryo-preserved crystals of MICA belong to the cubic space group F4(1)32 with lattice constants a = b = c = 260.7 Angstrom and diffract to a resolution limit of 3.0 Angstrom when cryo-preserved. These crystals do not diffract when handled conventionally.

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页码：451 / 453

页数：3

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