Thermographic visualization of cell death in tobacco and Arabidopsis

Pending cell death was visualized by thermographic imaging in bacterio-opsin transgenic tobacco plants, Cell death in these plants was characterized by a complex lesion phenotype, Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) (Chaerle et al. 1999 Nature Biotechnology 17, 813-816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of wafer evaporation from damaged cells. In analogy with previous observations on the-localized tobacco-TMV interaction (Chaerle et al. 1999), the kinetics of thermographic and continuous gas exchange measurements indicated that stomatal closure preceded tissue collapse, Localized spontaneous cell death could also be presymptomatically visualized in the Arabidopsis lsd2 mutant.