The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function

被引：183

作者：

Kim, Sung Eun ^{[2
]}

Song, Sang-Hun ^{[1
]}

Yun, Young Pil ^{[2
]}

Choi, Byung-Joon ^{[1
]}

Kwon, Il Keun ^{[3
,4
]}

Bae, Min Soo ^{[3
,4
]}

Moon, Ho-Jin ^{[3
,4
]}

Kwon, Yong-Dae ^{[1
]}

机构：

[1] Kyung Hee Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Seoul 130701, South Korea

[2] Korea Univ, Coll Med, Guro Hosp, Dept Orthoped Surg & Rare Dis, Seoul 152703, South Korea

[3] Kyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea

[4] Kyung Hee Univ, Sch Dent, Inst Oral Biol, Seoul 130701, South Korea

来源：

BIOMATERIALS | 2011年 / 32卷 / 02期

关键词：

Titanium; Heparin; Bone morphogenic protein 2 (BMP 2); Osteoblasts; Inflammation; Peri implantitis; MESENCHYMAL STEM-CELLS; LOW-MOLECULAR-WEIGHT; ACID-BASED HYDROGEL; ARTICULAR-CARTILAGE; SPINE FUSION; KAPPA-B; DEFECTS; DELIVERY; ADHESION; RHBMP-2;

D O I：

10.1016/j.biomaterials.2010.09.008

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface Ti surfaces were first modified by 3-amino-propyltriethoxysilane (ATPES) followed by grafting of heparin BMP-2 was then immobilized on the heparin-grafted Ti surfaces Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS) measurement of water contact angles and protein adsorption The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays measurement of alkaline phosphate (ALP) activity and determination of calcium deposition Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-alpha XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces respectively In addition Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti Furthermore BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti The inflammatory response was also decreased when cells were grown on heparin-grafted BMP-2-immobilized Ti surfaces The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces inflammation can be inhibited and osteoblast function promoted (C) 2010 Elsevier Ltd All rights reserved

引用

页码：366 / 373

页数：8

共 39 条

[1]

Increased osteoblast adhesion on nanograined Ti modified with KRSR [J].