Evaluation of two protocols of uremic rat model: Partial nephrectomy and infarction

被引:19
作者
Liu, ZC
Chow, KM
Chang, TMS [1 ]
机构
[1] McGill Univ, Fac Med, Artificial Cells & Organs Res Ctr, Montreal, PQ H3G 1Y6, Canada
[2] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China
关键词
5; 6; nephrectomy; partial nephrectomy; rat; resection; infarction; uremic model;
D O I
10.1081/JDI-120026028
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Animal models of chronic renal failure have been mostly achieved by partial ablation of renal parenchyma, the two most common techniques employed being surgical resection or infarction. Evaluation of the uremic model using these two techniques was carried out in Wistar rats. Two weeks after operative procedure, measured serum urea levels in the resection and infarction models were 59.1 and 64.3 mg/dL (normal range 15.6-24.4 mg/dL) respectively. However, the standard deviation in the former was significantly lower, 6.3 vs. 97.1 mg/dL from infarction model, p = 0.007. A consistent degree of glomerular filtration rate reduction was obtained in the resection model, resulting in 20-30% of normal creatinine clearance. This compared favorably with the creatinine clearance range (0.3-74% of normal) from the infarction model, in which two animals died of uremia and seven had higher than 50% of normal creatinine clearance. It is reasonable to attribute reproducibility and homogeneity demonstrated in the resection model to (i) more precise control of renal ablation extent with surgical techniques and (ii) less interplay of confounding injury mechanism to remnant kidney. These data support superiority of the resection model as an experimental toot for pathophysiological and or interventional investigations of chronic renal failure.
引用
收藏
页码:935 / 943
页数:9
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