Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2′-O-methyltransferase activity of nsp10/nsp16 complex

被引：61

作者：

Ke, Min ^{[1
,2
]}

Chen, Yu ^{[1
,2
]}

Wu, Andong ^{[1
,2
]}

Sun, Ying ^{[1
,2
]}

Su, Ceyang ^{[1
,2
]}

Wu, Hao ^{[1
,2
]}

Jin, Xu ^{[1
,2
]}

Tao, Jiali ^{[1
,2
]}

Wang, Yi ^{[1
,2
]}

Ma, Xiao ^{[1
,2
]}

Pan, Ji-An ^{[1
,2
]}

Guo, Deyin ^{[1
,2
]}

机构：

[1] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China

来源：

VIRUS RESEARCH | 2012年 / 167卷 / 02期

关键词：

SARS; Coronavirus; nsp10; nsp16; 2 '-O-methyltransferase; Peptides; ACUTE RESPIRATORY SYNDROME; METHYLTRANSFERASE; EXORIBONUCLEASE; REPLICATION; INHIBITORS; THERAPIES; REVEALS; GENOME; TARGET; VIRUS;

D O I：

10.1016/j.virusres.2012.05.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Coronaviruses are the etiological agents of respiratory and enteric diseases in humans and livestock, exemplified by the life-threatening severe acute respiratory syndrome (SARS) caused by SARS coronavirus (SARS-CoV). However, effective means for combating coronaviruses are still lacking. The interaction between nonstructural protein (nsp) 10 and nsp16 has been demonstrated and the crystal structure of SARS-CoV nsp16/10 complex has been revealed. As nsp10 acts as an essential trigger to activate the 2'-O-methyltransferase activity of nsp16, short peptides derived from nsp10 may have inhibitory effect on viral 2'-O-methyltransferase activity. In this study, we revealed that the domain of aa 65-107 of nsp10 was sufficient for its interaction with nsp16 and the region of aa 42-120 in nsp10, which is larger than the interaction domain, was needed for stimulating the nsp16 2'-O-methyltransferase activity. We further showed that two short peptides derived from the interaction domain of nsp10 could inhibit the 2'-O-methyltransferase activity of SARS-CoV nsp16/10 complex, thus providing a novel strategy and proof-of-principle study for developing peptide inhibitors against SARS-CoV. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：322 / 328

页数：7

共 28 条

[1] Critical residues of Semliki Forest virus RNA capping enzyme involved in methyltransferase and guanylyltransferase-like activities [J].