Enantioselective reduction of β-keto acids with engineered streptomyces coelicolor

被引：8

作者：

Booker-Milburn, KI

Gillan, R

Kimberley, M

Taguchi, T

Ichinose, K

Stephenson, GR

Ebizuka, Y

Hopwood, DA

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[3] Univ E Anglia, Sch Chem Sci & Pharm, Wolfson Mat & Catalysis Ctr, Norwich NR4 7TJ, Norfolk, England

[4] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 07期

关键词：

bioorganic chemistry; biosynthesis; enantioselectivity; enzymes; reduction;

D O I：

10.1002/anie.200462076

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Compelling evidence for the intermediacy of the free β-keto acid 1, rather than the corresponding enzyme-bound thiolate as previously proposed, in the biosynthesis of the antibiotic actinorhodin (2) was obtained from studies of the enantioselective reduction of a range of β-keto acids by the engineered strain of S. coelicolor CH999/pIJ5675. This excellent whole-cell biotransformation system gave the desired S β-hydroxy acids with >95% ee. © 2005 Wiley-VCH Verlag GmbH & Co. KCaA, Weinheim.

引用

页码：1121 / 1125

页数：5

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