Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase

被引：159

作者：

Bailey, Scott

Eliason, William K.

Steitz, Thomas A. ^{[1
]}

机构：

[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[2] Yale Univ, Howard Hughes Med Inst, New Haven, CT 06520 USA

[3] Yale Univ, Dept Chem, New Haven, CT 06520 USA

来源：

SCIENCE | 2007年 / 318卷 / 5849期

关键词：

D O I：

10.1126/science.1147353

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site.

引用

收藏

页码：459 / 463

页数：5

相关论文

共 32 条

[1] The crystal structure of the Thermus aquaticus DnaB helicase monomer [J].

Bailey, Scott ;

Eliason, William K. ;

Steitz, Thomas A. .

NUCLEIC ACIDS RESEARCH, 2007, 35 (14) :4728-4736

[2] Mapping protein-protein interactions within a stable complex of DNA primase and DnaB helicase from Bacillus stearothermophilus [J].

Bird, LE ;

Pan, H ;

Soultanas, P ;

Wigley, DB .

BIOCHEMISTRY, 2000, 39 (01) :171-182

[3] STRUCTURE AND FUNCTION OF ESCHERICHIA-COLI DNAB PROTEIN - ROLE OF THE N-TERMINAL DOMAIN IN HELICASE ACTIVITY [J].

BISWAS, SB ;

CHEN, PH ;

BISWAS, EE .

BIOCHEMISTRY, 1994, 33 (37) :11307-11314

[4] INTERACTIONS OF ESCHERICHIA-COLI PRIMARY REPLICATIVE HELICASE DNAB PROTEIN WITH SINGLE-STRANDED-DNA - THE NUCLEIC-ACID DOES NOT WRAP AROUND THE PROTEIN HEXAMER [J].

BUJALOWSKI, W ;

JEZEWSKA, MJ .

BIOCHEMISTRY, 1995, 34 (27) :8513-8519

[5] Identification of a region of Escherichia coli DnaB required for functional interaction with DnaG at the replication fork [J].

Chang, P ;

Marians, KJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (34) :26187-26195

[6] Regulation of bacterial priming and daughter strand synthesis through helicase-primase interactions [J].

Corn, Jacob E. ;

Berger, James M. .

NUCLEIC ACIDS RESEARCH, 2006, 34 (15) :4082-4088

[7] Crosstalk between primase subunits can act to regulate primer synthesis in trans [J].

Corn, JE ;

Pease, PJ ;

Hura, GL ;

Berger, JM .

MOLECULAR CELL, 2005, 20 (03) :391-401

[8] The arginine finger of bacteriophage T7 gene 4 helicase: Role in energy coupling [J].

Crampton, DJ ;

Guo, SY ;

Johnson, DE ;

Richardson, CC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (13) :4373-4378

[9] Mechanism of DNA translocation in a replicative hexameric helicase [J].

Enemark, Eric J. ;

Joshua-Tor, Leemor .

NATURE, 2006, 442 (7100) :270-275

[10] Unzipping mechanism of the double-stranded DNA unwinding by a hexameric helicase:: Quantitative analysis of the rate of the dsDNA unwinding, processivity and kinetic step-size of the Escherichia coli DnaB helicase using rapid quench-flow method [J].

Galletto, R ;

Jezewska, MJ ;

Bujalowski, W .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 343 (01) :83-99

← 1 2 3 4 →