Silica aerogels as supports for lipase catalyzed esterifications at sub- and supercritical conditions

被引：51

作者：

Novak, Z ^{[1
]}

Habulin, M ^{[1
]}

Krmelj, V ^{[1
]}

Knez, Z ^{[1
]}

机构：

[1] Univ Maribor, Fac Chem & Chem Engn, SI-2000 Maribor, Slovenia

来源：

JOURNAL OF SUPERCRITICAL FLUIDS | 2003年 / 27卷 / 02期

关键词：

silica aerogel; lipases; immobilization; supercritical CO2 drying;

D O I：

10.1016/S0896-8446(02)00233-4

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The enzymes (lipases from Candida rugosa and porcine pancreas) were immobilized on silica aerogels by sol-gel procedure followed by supercritical drying with CO2. Such immobilized enzymes were used as biocatalysts for esterification in supercritical CO2 and near critical propane at 40 degreesC and 100 bar. It was found out that the initial reaction rates in propane rose two to three times in comparison with the same reaction, catalyzed by free lipase. SC CO2 deactivated the non-immobilized lipase in reaction mixture while with the immobilized enzyme the conversion was 35%. The initial reaction rates in propane were 20 times higher than in water medium due to the properties of propane as a medium for esterification of fatty acids. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：169 / 178

页数：10

共 19 条

[1]

[Anonymous], 1999, CHEM SYNTHESIS USING

[2]

Bailey JE, 1986, BIOCHEMICAL ENG FUND

[3] Transglycosylation catalyzed by almond β-glucosidase and cloned Pichia etchellsii β-glucosidase II using glycosylasparagine mimetics as novel acceptors [J].