Pocket Epithelium in the Pathological Setting for HMGB1 Release

被引：43

作者：

Ebe, N. ^{[2
,3
]}

Hara-Yokoyama, M. ^{[1
,3
]}

Iwasaki, K. ^{[2
,4
]}

Iseki, S. ^{[5
]}

Okuhara, S. ^{[5
]}

Podyma-Inoue, K. A. ^{[1
,3
]}

Terasawa, K. ^{[1
]}

Watanabe, A. ^{[6
]}

Akizuki, T. ^{[2
]}

Watanabe, H. ^{[2
]}

Yanagishita, M. ^{[1
,3
]}

Izumi, Y. ^{[2
,3
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch, Div Biomatrix, Biochem Sect,Dept Hard Tissue Engn,Bunkyo Ku, Tokyo 1138549, Japan

[2] Tokyo Med & Dent Univ, Grad Sch, Div Biomatrix, Sect Periodontol,Dept Hard Tissue Engn,Bunkyo Ku, Tokyo 1138549, Japan

[3] Tokyo Med & Dent Univ, Int Res Ctr Mol Sci Tooth & Bone Dis, Global Ctr Excellence GCOE Program, Bunkyo Ku, Tokyo 1138549, Japan

[4] Tokyo Womens Med Univ, Dept Oral Maxillofacial Surg, Tokyo, Japan

[5] Tokyo Med & Dent Univ, Grad Sch, Dept Mol Craniofacial Embryol, Bunkyo Ku, Tokyo 1138549, Japan

[6] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Bunkyo Ku, Tokyo 1138549, Japan

来源：

JOURNAL OF DENTAL RESEARCH | 2011年 / 90卷 / 02期

关键词：

HMGB1; pocket epithelium; butyric acid; MOBILITY-GROUP BOX-1; CHROMATIN PROTEIN HMGB1; PERIODONTOPATHIC BACTERIA; CELL-DEATH; APOPTOSIS; ACID; PROLIFERATION; ACTIVATION; BUTYRATE; MEDIATOR;

D O I：

10.1177/0022034510385688

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

High-mobility group box-1 (HMGB1) protein acts as a transcription factor in the nucleus and also as a pro-inflammatory cytokine when released into extracellular fluids. The presence of higher levels of HMGB1 is reported in the gingival crevicular fluid from periodontal patients. Since the proliferation of bacteria within the periodontal pocket is closely involved in the exacerbation of periodontal disease, it is hypothesized that the periodontal pocket causes the release of HMGB1. Immunohistochemical staining of inflamed gingiva revealed that HMGB1 is exclusively dislocated from the nucleus to the cytoplasm in the pocket epithelium, whereas it is mainly present in the nucleus in the gingival epithelium. Butyric acid, an extracellular metabolite from periodontopathic bacteria populating the periodontal pocket, induced the passive release of HMGB1 as a result of eliciting necrosis in the human gingival epithelial cell line. Thus, the periodontal epithelium may provide a unique pathological setting for HMGB1 release by bacterial insult. Abbreviations: HMGB1, high-mobility group box-1; TNF-alpha, tumor necrosis factor-alpha; CHX, cycloheximide; PI, propidium iodide; ROS, reactive oxygen species; HDAC, histone deacetylase.

引用

页码：235 / 240

页数：6

共 32 条

[1]

HMGB1 is a potent trigger of arthritis [J].