Nucleic acid based biosensors: The desires of the user

被引:58
作者
Hahn, S [1 ]
Mergenthaler, S [1 ]
Zimmermann, B [1 ]
Holzgreve, W [1 ]
机构
[1] Univ Basel, Womens Hosp, Dept Res, Lab Prenatal Med, CH-4031 Basel, Switzerland
关键词
DNA biosensor diagnosis; PCR microarray;
D O I
10.1016/j.bioelechem.2004.07.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The need for nucleic acid based diagnostic tests has increased enonnously in the last few years. On the one hand, this has been stimulated by the discovery of new hereditary genetic disease loci following the completion of the Human Genome Project, but also by the presence of new rapidly spreading viral threats, such as that of the SARS epidemic, or even micro-organisms released for the purpose of biological warfare. As in many instances rapid diagnoses of specific target genetic loci is required, new strategies have to be developed, which will allow this to be achieved directly at the point-of-care setting. One of these avenues being explored is that of biosensors. In this review, we provide an overview of the current state of the art concerning the high-throughput analysis of nucleic acids, and address future requirements, which will hopefully be met by new biosensor-based developments. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:151 / 154
页数:4
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