DNA sequence recognition by bis-linked netropsin and distamycin derivatives

被引：36

作者：

Grokhovsky, SL

Surovaya, AN

Burckhardt, G

Pismensky, VF

Chernov, BK

Zimmer, C ^{[1
]}

Gursky, GV

机构：

[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 117984, Russia

[2] Univ Jena, Inst Mol Biol, D-6900 Jena, Germany

[3] Univ Oslo, Ctr Med Studies, Oslo, Norway

来源：

FEBS LETTERS | 1998年 / 439卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

DNA-drug interaction; distamycin; bis-netropsin; bis-distamycin; d(TTTTAAAA) sequence;

D O I：

10.1016/S0014-5793(98)01379-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the interaction of cis-diammine Pt(II)-bridged bis-netropsin, cis-diammine Pt(II)-bridged bis-distamycin and oligomethylene-bridged bis-netropsin with synthetic DNA fragments containing pseudosymmetrical AT-rich nucleotide sequences and compared it with the interaction of the parent compounds netropsin and distamycin A, For fragments containing multiple blocks of (A/T)(4) and (T/A)(4) separated by zero, one, two and three GC-base pairs, DNase I footprinting and CD spectroscopy studies reveal that 5'-TTTTAAAA-3' is the strongest affinity binding site for cis-diammine Pt(II)-bridged bis-netropsin and bis-distamycin, They both bind less strongly to a DNA region containing the sequence 5'-AAAATTTT-3'. Netropsin, distamycin A and oligomethylene-bridged bis-netropsin exhibit far less sequence discrimination. (C) 1998 Federation of European Biochemical Societies.

引用

页码：346 / 350

页数：5

共 30 条

[1] DNA-SEQUENCE PREFERENCES OF SEVERAL AT-SELECTIVE MINOR-GROOVE BINDING LIGANDS [J].