Identification of a mitochondrial external NADPH dehydrogenase by overexpression in transgenic Nicotiana sylvestris

被引:68
作者
Michalecka, AM
Agius, SC
Moller, IM
Rasmusson, AG
机构
[1] Lund Univ, Dept Cell & Organism Biol, SE-22362 Lund, Sweden
[2] Riso Natl Lab, Plant Res Dept, DK-4000 Roskilde, Denmark
关键词
alternative oxidase; NAD(P)H dehydrogenase; plant mitochondria; respiratory chain; transgenic plants; uncoupling protein;
D O I
10.1046/j.1365-313X.2003.01970.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plant respiratory chain contains a complex setup of non-energy conserving NAD(P)H dehydrogenases, the physiological consequences of which are highly unclear. An expression construct for the potato (Solanum tuberosum L., cv. Desiree) ndb1 gene, a homologue of bacterial and fungal type II NAD(P)H dehydrogenases, was introduced into Nicotiana sylvestris. Transgenic lines with high transcript and protein levels for St-NDB1 had up to threefold increased activity of external NADPH dehydrogenase in isolated mitochondria as compared to the wild type (WT). In two lines, the external NADPH dehydrogenase activity was instead 10-fold decreased, indicating that the corresponding N. sylvestris gene had been suppressed. Activities of external and internal rotenone-insensitive NADH dehydrogenases were unchanged in the transgenic lines. The results demonstrate that the St-ndb1 encodes an external dehydrogenase specific for NADPH and dependent on calcium for activity. Transgenic lines overexpressing St-ndb1 had specifically increased protein levels for alternative oxidase and uncoupling protein, as compared to the WT and one co-suppressing line. This indicates cross-talk in the expressional control, or metabolic conditions influencing it, for the different categories of energy-dissipating proteins that bypass oxidative phosphorylation. The potential effects of external NADPH oxidation on other cellular processes are discussed.
引用
收藏
页码:415 / 425
页数:11
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