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Regulation of SNARK activity in response to cellular stresses

被引：43
作者：
Lefebvre, DL
Rosen, CF
机构：
[1] Univ Toronto, Toronto Gen Hosp, Dept Med, Div Dermatol,Banting Inst, Toronto, ON M5G 1L5, Canada
[2] Toronto Western Hosp, Toronto, ON M5G 1L5, Canada
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2005年 / 1724卷 / 1-2期
关键词：
protein kinase; SNARK; AMPK; stress; metformin;
D O I：
10.1016/j.bbagen.2005.03.015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SNARK is a member of the AMPK subfamily of serine/threonine protein kinases. In this study, we examined the regulation of SNARK activity in kidney (BHK, HEK293), pancreatic beta-cell insulinoma. (INS-1), hepatocarcinoma (H411E) and keratinocyte (NRKC)-derived cell lines in response to diverse cellular stresses. We show that SNARK activity is regulated by glucose- or glutamme-deprivation, induction of endoplasmic reticulum stress by homocysteine or DTT, elevation of cellular AMP and/or depletion of ATP, hyperosmotic stress, salt stress, ultraviolet B radiation and oxidative stress caused by hydrogen peroxide. Moreover, the regulation of SNARK activity in response to cellular stresses depends greatly upon cell type. Furthermore, SNARK activity is downregulated by metformin in a dose- and time-dependent manner in H411E cells. These observations support a role for SNARK as a molecular component of the cellular stress response. (c) 2005 Elsevier B.V All rights reserved.
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页码：71 / 85
页数：15
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