Binding of interleukin-8 to heparan sulphate enhances cervical maturation in rabbits

被引:7
作者
Belayet, HM [1 ]
Kanayama, N [1 ]
Khatun, S [1 ]
Sumimoto, K [1 ]
Kobayashi, T [1 ]
Terao, T [1 ]
机构
[1] Hamamatsu Univ Sch Med, Dept Obstet & Gynecol, Hamamatsu, Shizuoka 4313192, Japan
关键词
cervical maturation; heparan sulphate; hyaluronic acid; interleukin-8; neutrophil activation;
D O I
10.1093/molehr/5.3.261
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cervical ripening is a cytokine-trigged process with substantial remodelling of the cervical extracellular matrix. Interleukin-8 (IL-8) is an important cytokine in cervical maturation, Glycosaminoglycans are also included in this process, but their role in not clearly understood. The effects of heparan sulphate (HS), hyaluronic acid (HA), IL-8, HS + IL-8 and HA + IL-8 on biochemical properties of the cervix were examined in non-pregnant rabbits, The changes in vascular pattern with collagen structure of the cervices and immunohistochemical studies, together with the relative collagen concentrations, were determined. A reduction in relative collagen concentration was significant after HS + IL-8,IL-8 and HA + IL-8 treatment (all P < 0.0001). Gel electrophoresis analysis showed that IL-8 bound preferentially to HS than to HA. Neutrophils were significantly increased in number (P < 0.0001) and located predominantly beneath the glandular epithelium and around the blood vessels after HS + IL-8 treatment, HS + IL-8 treatment caused cervices to increase their water content and become oedematous. The collagen fibres were considerably dissociated, the interfibrillar spaces markedly dilated, and the blood vessels notably increased and dilated. We conclude that binding to HS enhances the activity of IL-8 in inducing cervical maturation.
引用
收藏
页码:261 / 269
页数:9
相关论文
共 49 条
[1]   Interleukin-8 expression and modulation in human preovulatory follicles and ovarian cells [J].
Arici, A ;
Oral, E ;
Bukulmez, O ;
Buradagunta, S ;
Engin, O ;
Olive, DL .
ENDOCRINOLOGY, 1996, 137 (09) :3762-3769
[2]   Amniotic fluid interleukins in Swedish and Mozambican pregnant women [J].
Axemo, P ;
Brauner, A ;
Pettersson, M ;
Eriksson, L ;
Rwamushaija, E ;
Bergstrom, S .
GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 1996, 41 (02) :113-117
[3]   INTERLEUKIN-8 PRODUCTION BY THE HUMAN CERVIX [J].
BARCLAY, CG ;
BRENNAND, JE ;
KELLY, RW ;
CALDER, AA .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1993, 169 (03) :625-632
[4]   LEUKOCYTE-ENDOTHELIAL CELL RECOGNITION - 3 (OR MORE) STEPS TO SPECIFICITY AND DIVERSITY [J].
BUTCHER, EC .
CELL, 1991, 67 (06) :1033-1036
[5]   VARIATIONS IN THE DISTRIBUTION OF GLYCOSAMINOGLYCANS IN THE UTERINE CERVIX OF THE PREGNANT WOMAN [J].
CABROL, D ;
BRETON, M ;
BERROU, E ;
VISSER, A ;
SUREAU, C ;
PICARD, J .
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1980, 10 (05) :281-287
[6]  
CALDER AA, 1981, CERVIX PREGNANCY LAB, P103
[7]  
Caplan AI, 1980, DILATATION UTERINE C, P79
[8]   Cervical ripening in guinea-pigs after a local application of nitric oxide [J].
Chwalisz, K ;
Shao-Qing, S ;
Garfield, RE ;
Beier, HM .
HUMAN REPRODUCTION, 1997, 12 (10) :2093-2101
[9]   CERVICAL RIPENING WITH THE CYTOKINES INTERLEUKIN-8, INTERLEUKIN-1-BETA AND TUMOR-NECROSIS-FACTOR-ALPHA IN GUINEA-PIGS [J].
CHWALISZ, K ;
BENSON, M ;
SCHOLZ, P ;
DAUM, J ;
BEIER, HM ;
HEGELEHARTUNG, C .
HUMAN REPRODUCTION, 1994, 9 (11) :2173-2181
[10]   EFFECT OF PREGNANCY AND LABOR ON HUMAN CERVIX - CHANGES IN COLLAGEN, GLYCOPROTEINS, AND GLYCOSAMINOGLYCANS [J].
DANFORTH, DN ;
VEIS, A ;
BREEN, M ;
WEINSTEIN, HG ;
BUCKINGHAM, JC ;
MANALO, P .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1974, 120 (05) :641-651