Variations in DNA methylation patterns during the cell cycle of HeLa cells

被引：51

作者：

Brown, Shelley E. ^{[1
]}

Fraga, Mario F. ^{[3
]}

Weaver, Ian C. G. ^{[2
]}

Berdasco, Maria ^{[3
]}

Szyf, Moshe ^{[1
]}

机构：

[1] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada

[2] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ H3G 1Y6, Canada

[3] Spanish Natl Canc Ctr, Program Mol Pathol, Canc Epigenet Lab, Madrid, Spain

来源：

EPIGENETICS | 2007年 / 2卷 / 01期

关键词：

DNA methylation; epigenetics; CpG microarray; cell cycle; cancer; repetitive sequences;

D O I：

10.4161/epi.2.1.3880

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation has been viewed as a stable component of the epigenome, established during development and fixed thereafter. Here we have found that the DNA methylation pattern varies during a single cell cycle, with the global levels of DNA methylation decreased in G(1) and increase during S phase. There was little change in the DNA methylation levels in repetitive sequences throughout the cell cycle. However using a human CpG island microarray it was revealed that 174 CG-containing sequences were differentially methylated between G1 and S. Seventy-five percent of all the variations in DNA methylation detected in unique sequences represented hypomethylation at G(0), with changes occurring in both CpG islands and non-CpG islands. This is the first demonstration of a dynamic DNA methylation pattern within a single cell cycle of a mature somatic cell. These data are important for our understanding of the stability of DNA methylation patterns in somatic cells.

引用

页码：54 / 65

页数：12

共 74 条

[1] CPG ISLANDS - FEATURES AND DISTRIBUTION IN THE GENOMES OF VERTEBRATES [J].

AISSANI, B ;

BERNARDI, G .

GENE, 1991, 106 (02) :173-183

[2] Concurrent replication and methylation at mammalian origins of replication [J].

Araujo, FD ;

Knox, JD ;

Szyf, M ;

Price, GB ;

Zannis-Hadjopoulos, M .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (06) :3475-3482

[3] The human RNA surveillance factor UPF1 is required for S phase progression and genome stability [J].

Azzalin, CM ;

Lingner, J .

CURRENT BIOLOGY, 2006, 16 (04) :433-439

[4] A mammalian protein with specific demethylase activity for mCpG DNA [J].

Bhattacharya, SK ;

Ramchandani, S ;

Cervoni, N ;

Szyf, M .

NATURE, 1999, 397 (6720) :579-583

[5] Serum deprivation and protein synthesis inhibition induce two different apoptotic processes in N18 neuroblastoma cells [J].

Boix, J ;

Fibla, J ;

Yuste, VJ ;

Piulats, JM ;

Llecha, N ;

Comella, JX .

EXPERIMENTAL CELL RESEARCH, 1998, 238 (02) :422-429

[6] Myc represses transcription through recruitment of DNA methyltransferase corepressor [J].

Brenner, C ;

Deplus, R ;

Didelot, C ;

Loriot, A ;

Viré, E ;

De Smet, C ;

Gutierrez, A ;

Danovi, D ;

Bernard, D ;

Boon, T ;

Pelicci, PG ;

Amati, B ;

Kouzarides, T ;

de Launoit, Y ;

Di Croce, L ;

Fuks, F .

EMBO JOURNAL, 2005, 24 (02) :336-346

[7] Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases [J].

Brueckner, B ;

Boy, RG ;

Siedlecki, P ;

Musch, T ;

Kliem, HC ;

Zielenkiewicz, P ;

Suhai, S ;

Wiessler, M ;

Lyko, F .

CANCER RESEARCH, 2005, 65 (14) :6305-6311

[8] Demethylase activity is directed by histone acetylation [J].

Cervoni, N ;

Szyf, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (44) :40778-40787

[9] Inhibition of mixed-lineage kinase (MLK) activity during G2-phase disrupts microtubule formation and mitotic progression in HeLa cells [J].

Cha, HJ ;

Dangi, S ;

Machamer, CE ;

Shapiro, P .

CELLULAR SIGNALLING, 2006, 18 (01) :93-104

[10] PARENTAL-SPECIFIC METHYLATION OF AN IMPRINTED TRANSGENE IS ESTABLISHED DURING GAMETOGENESIS AND PROGRESSIVELY CHANGES DURING EMBRYOGENESIS [J].

CHAILLET, JR ;

VOGT, TF ;

BEIER, DR ;

LEDER, P .

CELL, 1991, 66 (01) :77-83

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