Development of a genetic manipulation system for Haemophilus parasuis

被引：42

作者：

Bigas, A

Garrido, ME

de Rozas, AMP

Badiola, I

Barbé, J

Llagostera, M ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Barcelona, Spain

[2] Univ Autonoma Barcelona, IRTA, CReSA, Bellaterra 08193, Barcelona, Spain

来源：

VETERINARY MICROBIOLOGY | 2005年 / 105卷 / 3-4期

关键词：

Haemophilus parasuis; DNA transformation; knockout mutants; genetic manipulation;

D O I：

10.1016/j.vetmic.2004.10.015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Haemophilus parasuis is a member of the family Pasteurellaceae and an important respiratory-tract pathogen of swine, which is the etiological agent of Glasser's disease. Because no genetic manipulation system is available for H. parasuis so far, in vivo studies about the role of its genes involved in virulence are unfeasible. Here we demonstrate that H. parasuis has a cyclic AIMP (cAMP)-dependent natural transformation system that enables the uptake of DNA in which the ACCGAACTC sequence signal must be present. After improving DNA transformation parameters, such as cAMP and DNA concentration and exposition time of the exogenous DNA, a knockout mutant of H. parasuis defective in the thy gene, encoding the thymidylate synthase enzyme. has been constructed. Data presented in this work open the possibility for the functional analysis of genes involved in the infectious process of this animal pathogen. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：223 / 228

页数：6

共 21 条

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