Phosphatidylinositol 3,5-Bisphosphate (PI(3,5)P2) Potentiates Cardiac Contractility via Activation of the Ryanodine Receptor

被引:27
作者
Touchberry, Chad D. [1 ]
Bales, Ian K. [1 ]
Stone, Jessica K. [1 ]
Rohrberg, Travis J. [1 ]
Parelkar, Nikhil K. [1 ]
Nguyen, Tien [1 ]
Fuentes, Oscar [3 ]
Liu, Xia [4 ]
Qu, Cheng-Kui [4 ]
Andresen, Jon J. [1 ,2 ]
Valdivia, Hector H. [5 ]
Brotto, Marco [1 ,2 ]
Wacker, Michael J. [1 ,2 ]
机构
[1] Univ Missouri, Muscle Biol Res Grp, Sch Med, Kansas City, MO 64108 USA
[2] Univ Missouri, Muscle Biol Res Grp, Sch Nursing, Kansas City, MO 64108 USA
[3] Univ Bio Bio, Dept Ciencias Basicas, Chillan 3780000, Chile
[4] Case Western Reserve Univ, Sch Med, Cleveland, OH 44106 USA
[5] Univ Wisconsin, Sch Med & Publ Hlth, Madison, WI 53711 USA
基金
美国国家卫生研究院;
关键词
BINDING; PIP2;
D O I
10.1074/jbc.M110.179689
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is the most recently identified phosphoinositide, and its functions have yet to be fully elucidated. Recently, members of our muscle group have shown that PI(3,5)P2 plays an important role in skeletal muscle function by altering Ca2+ homeostasis. Therefore, we hypothesized that PI(3,5)P2 may also modulate cardiac muscle contractility by altering intracellular Ca2+ ([Ca2+](i)) in cardiac myocytes. We first confirmed that PI(3,5)P2 was present and increased by insulin treatment of cardiomyocytes via immunohistochemistry. To examine the acute effects of PI(3,5)P2 treatment, electrically paced left ventricular muscle strips were incubated with PI(3,5)P2. Treatment with PI(3,5)P2 increased the magnitude of isometric force, the rate of force development, and the area associated with the contractile waveforms. These enhanced contractile responses were also observed in MIP/Mtmr14(-/-) mouse hearts, which we found to have elevated levels of PI(3,5)P2. In cardiac myocytes loaded with fura-2, PI(3,5)P2 produced a robust elevation in [Ca2+](i). The PI(3,5)P2-induced elevation of [Ca2+](i) was not present in conditions free of extracellular Ca2+ and was completely blocked by ryanodine. We investigated whether the phosphoinositide acted directly with the Ca2+ release channels of the sarcoplasmic reticulum (ryanodine receptors; RyR2). PI(3,5)P2 increased [H-3] ryanodine binding and increased the open probability (P-o) of single RyR2 channels reconstituted in lipid bilayers. This strongly suggests that the phosphoinositide binds directly to the RyR2 channel. Thus, we provide inaugural evidence that PI(3,5)P2 is a powerful activator of sarcoplasmic reticulum Ca2+ release and thereby modulates cardiac contractility.
引用
收藏
页码:40312 / 40321
页数:10
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