Use of fluorescent probes to follow membrane traffic in nerve terminals

被引:8
作者
Guatimosim, C [1 ]
Romano-Silva, MA [1 ]
Gomez, MV [1 ]
Prado, MAM [1 ]
机构
[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Farmacol, Lab Neurofarmacol, BR-31270901 Belo Horizonte, MG, Brazil
关键词
laser scanning confocal microscopy; FM1-43; exocytosis; synaptic vesicles; endocytosis;
D O I
10.1590/S0100-879X1998001100018
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals.
引用
收藏
页码:1491 / 1500
页数:10
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