Heptameric association of light-harvesting complex II trimers in partially solubilized photosystem II membranes

被引：55

作者：

Dekker, JP

van Roon, H

Boekem, EJ

机构：

[1] Univ Groningen, Groningen Biotechnol & Biomol Sci Inst, NL-9747 AG Groningen, Netherlands

[2] Vrije Univ Amsterdam, Inst Mol Biol Sci, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands

来源：

FEBS LETTERS | 1999年 / 449卷 / 2-3期

关键词：

photosystem II; light-harvesting complex II; heptamer; electron microscopy;

D O I：

10.1016/S0014-5793(99)00442-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of seven trimeric light-harvesting complex II proteins, The complex was readily observed in partially-solubilized Tris-washed photosystem II membranes from spinach but was also found to occur, with a low frequency, in oxygen-evolving photosystem II membranes. The structure reveals sis peripheral trimers with the same rotational orientation and a central trimer with the opposite orientation. We conclude that the heptamer represents a naturally occurring aggregation state of part of the light-harvesting complex II trimers in the thylakoid membranes. (C) 1999 Federation of European Biochemical Societies.

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页码：211 / 214

页数：4

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