Effect of molecular mass of methoxypoly(ethylene glycol) activated with succinimidyl carbonate on camouflaging pancreatic islets

被引：6

作者：

Barani, Leila ^{[1
]}

Vasheghani-Farahani, Ebrahim ^{[1
]}

Lazarjani, Hamideh Aghajani ^{[1
]}

Hashemi-Najafabadi, Sameereh ^{[1
]}

Atyabi, Fatemeh ^{[2
]}

机构：

[1] Tarbiat Modares Univ, Fac Chem Engn, Biotechnol Grp, Tehran 1411713116, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Dept Pharmaceut, Tehran, Iran

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2010年 / 57卷

关键词：

insulin; lymphocyte; methoxypoly(ethylene glycol) (mPEG); succinimidyl carbonate-activated methoxypoly(ethylene glycol) (mPEG-SC); pancreatic islet; PEGylation; POLYETHYLENE-GLYCOL; CELLS;

D O I：

10.1042/BA20100145

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The surface modification of Langerhans islets by grafting activated poly(ethylene glycol) on to their capsules in order to prevent immune-system stimulation is a novel method in diabetes cell therapy. In the present study, mPEG [methoxypoly(ethylene glycol)] with two molecular masses of 5 and 10 kDa, activated with SC (succinimidyl carbonate), was grafted on to the surface of pancreatic islets at a polymer concentration of 22 mg/ml. It was found that PEGylated islets were viable and active, and no morphological changes on the collagen capsule of islets were observed. The amount of interleukin-2 secretion from lymphocytes co-cultured with islets PEGylated with mPEG-SC of 5 and 10 kDa was 112.12 +/- 23.09 pg/ml and 172.75 +/- 27.94 pg/ml respectively. Thus mPEG-SC (SC-activated mPEG) with higher molecular mass was more suitable for camouflaging islets from the immune system.

引用

页码：25 / 30

页数：6

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